黄芪总苷通过抑制P38通路缓解氧糖剥夺/复氧后的星形胶质细胞水肿并降低AQP4表达

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Abstract：ObjectiveToinvestigatetheefectoftotalAstragalussaponins （AST）onastrocyteswelinginducedbyglucoseoxygen deprivation and reoxygenation （OGD/R）and its molecular mechanism.Methods Primary astrocytes isolated from he brainof postnatalday2SDratswere treatedwithsolventvehicle，AST，orASTcombinedwithU-46619（ap38activator） 24h priortoandringexposuretoGD/R.Theangesincellumeandmorphologywereobsered，andintracelllaractive oxygen species （ROS）levelsand lactate dehydrogenase （LDH）activity were detected.The expressonof aquaporin 4 （AQP4） oncell membranewasobservedusing immunofluorescenceconfocalmicroscopy，and Western bloting wasperformedto detectthechanges inp38signaling pathwayphosphorylation levelsandAQP4 expresson level.ResultsASTtreatment significantlyreducedOGD/R-inducedincreaseinastrocytevolumeROlevelsandLDHrelease.TheAT-treatedcellsalso exhibitedimprovedcellmorphologyrducedaggregationofAQP4onthecellmembrane，anddecreasedp38phosphorlation andAQP4expression levels.TheprotectiveeffectsofASTagainst OGD/R-inducedcellinjuries were significantlyatteuated by combined treatment of the cels with U-46619. Conclusion AST alleviate astrocyte sweling induced by OGD/R injury by inhibiting the activation of the p38 signaling pathway and AQP4 overexpression on the cell membrane.

Keywords： total Astragalus glycosides;astrocytesweling;aquaporin4; p38 signaling pathway;oxygen-glucose deprivation/ reoxygenation

缺血性脑卒中是一种常见的神经系统疾病，其特点为病情进展迅速，且具有高致死率和致残率[1。（剩余14118字）