益气解毒方通过调控AKT1/GLUT1信号通路抑制鼻咽癌的恶性进展

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Abstract：ObjectiveToinvestigatetheroleoftheAKT1/GLUT1signalingpathwayinmediatingtheinhibitoryefectofYiqi JieduFormula（YQJDF）againstnasopharyngealcarcinoma （NPC）cels.MethodsMoleculardockingwasemployedtoanalyze thebindingaityetweethactiecomponentsofadAK1.ssayaltecellalysis woud healingassayandMatrigel invasion chamber assay were used to evaluate theefect of YQJDF extract on proliferation， migration and invasionabiliesofumanNPCcellines5-8Fand6-1B，andthechanges incelular protein expressionlevels weredetected using Western blotting.Ina BALB/cnude mouse modelbearing NPCcellxenografts，tumor growth were observed following treatment with daily gavage with normal salineor YQJDF extract （15.357g/kg） for18 consecutive days or withintraperitonealinjectionsof5-Fueveryotherday.ThechangesintheexpresionsofAKT/GLUTsignalingaisproteins inthexenograftswereexaminedusingWestern bloting.Results IntheNPCcellines，treatment with YQJDFextract significantlidcllfgosdedpsof N-cadherinandvimentin.TeapplicationofGLUorAKactivatorspartialleversedtheibitoryefetsofQJDFon the NPC cels. In the tumor-bearing mouse models， treatment with YQJDF extract obviously suppresed tumor growth and down-regulated the expressionofAKT，p-AKTandGLUT1 inthetumor tissues.Conclusion YQJDF inhibitsproliferation， invasion，andmigrationof NPCcellsbyinhibiting the AKT1/GLUT1 signaling pathway.

Keywords：nasopharyngealcarcinoma;YiqiJieduFormula;AKT1/GLUT1signalingpathway;invasionandmigration

鼻咽癌（NPC）是起源于鼻咽上皮的恶性肿瘤，与遗

传因素、微生物感染（EB病毒等）环境、饮食及社会因素密切相关。（剩余14387字）