异牡荆素通过促进SIRT3表达减轻糖尿病小鼠的心肌氧化应激损伤

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Abstract：ObjectiveToinvestigatetheprotectiveefectofsovitexin（ISO）onthemyocardiumofdiabeticmiceandexploreits mechanism.Methods Eighteenadult male C57mice wererandomly divided intocontrol group，diabetes melitus （DM） group and DM+ISO group （n=6）.Primary cultures of neonatal mouse cardiomyocytes （NMCMs）were exposed to high glucose （33mmol/L） andtreated withISOaloneor incombination with 3-TYP（a SIRT3 inhibitor）.HE staining was used toevaluate myocardial injuryinthediabeticmice，andthelevelsofMDA，SODandGSH- ⋅Px inthemyocardiumweredetectedwith ELISA.The expressionsof iNOs，NOX2and8-OHdG weredetected using immunoistochemistryandfluorescencestaining. WesternblotingwasusedtoanalyzetheexpressionlevelsofNRF2，NOX2，NQO1andST3andROSlevelswere determinedwithflowcytometry.ResultsThediabeticmiceshowedobviousinflammatorycellinfiltrationinthemyocardium， increasedmyocardiallevelsofIL-1β，IL-6andTNF ⋅α， decreasedexpressionlevelsofNQO1，NRF2andRT3proteins，d increasedexpressionlevelsofNOX2andAC-SOD2proteins.ISOtreatmentof thediabeticmicesignificantlyreduced myocardial inflammatorycell infiltration，lowered the levelsof IL-1β，IL-6and TNF- ⋅α， restored the protein levels of NQO1， NRF2 and SIRT3，and decreased theprotein levels of NOX2 and AC-SOD2. Conclusion ISOcan aleviate diabetic myocardial injuryinmice bypromoting SIRT3 expressionand reducing oxidative stress.

Keywords： isovitexin;diabetesmelitus; cardiomyopathy;oxidative stress;SIRT3

糖尿病患病率正在逐年升高，糖尿病患者极易发展为糖尿病心肌病（DCM，表现为糖尿病患者在无明显冠心病，高血压等其他心脏疾病的情况下出现的与糖尿病相关的炎症，线粒体功能障碍，氧化应激引起的心肌结构和功能异常[2]。（剩余16303字）