肝细胞癌中MPP6高表达预测患者不良预后并促进肿瘤恶性生物学行为

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Abstract： Objective Toinvestigate theexpressonof membrane proteinpalmitoylated6（MPP6）inhepatocelluarcarcinoma （HCC）andanalyzeitscorelationwithclinicopathologicalfeaturesandpatientprognosisanditsimpactonbiological behaviors of HCCcels.Methods We examined MPP6 expressons using immunohistochemistry in tumorand adjacenttissues from118HCCpatientsundergoingsurgeriesinour hospitalfromJanuary2017toDecember，2019，andanalyzedthe correlationofMPP6expresionlevelswithclinicopathologicaldataandoverallsurvival （OS）ofthepatientsusingCox regressionanalysis.Inhuman hepatoma Hep3Bcels，，theefectsoflentivirus-mediatedMPP6knockdownonbiological behaviorsoflleaadingoosyoagsyaelldl Results Compared withadjacent tissues，HCC tissues showed significant overexpression of MPP6 （P<0.001） ，whose expression levels was correlated with liver cirrhosis（ （P=0.028） and baseline albumin level （P=0.035） of the patients. Survival analysis suggested that HCC patients with a high expression of MPP6 had a worse prognosis （P<0.001） ; Cox regression analysis indicated that MPP6 expression （P=0.012， HR： 2.335， 95% CI： 1.502-3.629） was an independent predictor of OS of HCC patients followinghepatectoy.InHep3Bcells，6nockdownobviouslyiibiedcellproliferation，gratonadsion P< 0.05）and caused cell cycle arrest in Go/G1 phase （P<0.001） .Conclusion MPP6 is highly expressed in HCC tissues and correlates with importantclinicopathological features and unfavorableprognosisof the patients.MPP6knockdowninhibits proliferation， migration and invasion and blocks cellcycle progression in Go/G1 phase.

Keywords： hepatocelular carcinoma; membrane protein palmitoylated 6; prognosis; malignantbiological behaviors

原发性肝癌（PLC）是常见的消化系统恶性肿瘤，其在全球和中国癌症相关死亡原因中均位居第2位[12]。（剩余11710字）