化瘀通便汤通过抑制PI3K/Akt信号通路促进慢传输型便秘大鼠肠道Cajal间质细胞的胞葬作用

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Abstract： Objective Toexplore the mechanism of Huayu Tongbian Decoction （HYTBD）forrelieving slowtransitconstipation （STC） inrats.Methods Sixty SDratmodels of STC induced by intragastric administration of loperamide hydrochloride suspension wererandomizedequallyinto5groupsfor treatment withdaily gavage withsaline （STCmodel group），low-， medium-，orighdoseHBD，ormosapride（positivecontrolgroup），withanother12normalratsreceivingsalinegavageas theblankcontrolgroup.Thfirstblackstooltime，fecalwatercontentandintestinalpropulsionrateoftheratsweremeasured， andthe ultrastructureofinterstitialCajal cels （ICCs）wasobserved using transmission electron microscopytocalculate the mitochondrialvacuolarrate.Thephagocytosisrateofmacrophages was detected usingimmunofluorescence assayandcolonic pathologieswereexainedsingHEstaing.eoloicexpresionlevelsof-KitCD47，3KandAktweredeectedith immunohistochemistryorWestenBlotingandtheircorrelationswitheferocytosiswereanalyzed.ResultsTheratodelsof STCshowed significantultrastructural damageoftheICCs withincreasedfirstblack stooltime，mitochondrialvacuolarrate， andcolonic expresionlevelsofCD47，p-PI3K/PI3Kandp-Akt/Aktproteis，loweredfecalwatercontent，intestialproulsion rateandc-Kitxpesonlel，itoutigiantangesingyicate.eatmentwithspeialleigh dose，significtlyrsdealteetstialsiategotosteolicKitpod decreasedthefistblackstooltime，mitochondrialvacuolarrateandcolonicexpreionsofCD47，p-PIK/PI3Kandp-AtAkt inthe mouse models.Conclusion HYTBDimproves STC inrats possibly by down-regulating colonic CD47 expressionand improving macrophagephagocytosisrateviainhibitingPI3KandAktphosphorylation，therebypromoting efferocytosisofthe ICCs.

Keywords： Huayu Tongbian Decoction; eferocytosis;slowtransitconstipation; PI3K/Aktsignalingpathway;interstitialCjal cells

慢传输型便秘（STC）是由于肠道传输无力导致肠内容物排出缓慢而引起的疾病，结肠内肠内容物移动迟缓、粪便干结以及患者排便次数明显减少均为常见的临床表现，多种治疗方法效果不明显，严重者甚至需要手术干预[1-3]。（剩余12659字）