基于液相色谱-质谱串联法技术的32种类固醇激素的检测方法建立及其在妊娠全周期变化应用

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Abstract：ObjectiveToestablishahighlysensitiveandspecificliquidchromatography-tandemmassspectrometry（C-MS/ MS）-basedanalyticalmethodfordetectingsteroidhormonesinhumanserum.MethodsSolid-phaseextraction （SPE）wasused forsamplepretreatment.Chromatographicconditionswereoptimized toachievebaselineseparation，andmassspectrometry parameters wereadjusted toensureaccurate detection of traceamounts of hormones.After validation of thelinear range， accuracy，and precision，the method wasapplied indetectionofserum samples from normalpregnant women foranalyzing dynamicvarationcaracteristicsf32seroidomosduringteist，cond，andtirdrimestersofpregnancyults The linear range of the method was 0.001-7500ng/mL， with a limit of detection （LOD） of 0.0007ng/mL . Both intra-day and inter-day coefficients of variation （CV） of the method were less than 10% ，the spiked recovery ranged from 86.21% to 112.67% the extraction efficiency was 85.05% 106.3%， and the matrix effects were 85.60%-113.22% . The results of detection using this methodfor serum steroid hormones duringpregnancyrevealed significant elevationof mostof thehormones（suchas11- deoxycortisol，1β-royprogesterone，dehdroepandrosteronesulfatadestriol）iththeprogreioofcy indicatingaclosecorrelationwiththedevelopmentoffetal-placentalfunction.Somehormonesincreasedinthethirdtrimester possiblydue tomaternaladaptiveregulationand maturationoffetaladrenal function.Conclusion Thedetectionresults using themethodestablishedinthisstudyrevealdynamicvariationsofsteroidhormonesduringpregnancywhichprovidesues forfurther physiological and pathological studies of these hormones.

Keywords：steroid hormones; liquid chromatography-tandemmassspectrometry;solid-phase extraction

类固醇激素是由性腺与肾上腺合成的胆固醇衍生而来的脂溶性化合物，胆固醇经一系列酶促反应转化为盐皮质激素、糖皮质激素、雌激素和雄激素等类固醇激素[1。（剩余18467字）