瓦雷兹芽孢杆菌FZB42中DUF1453家族蛋白CcdC的生物信息学分析及对菌株生长发育的影响

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中图分类号：Q51 文献标志码：A 文章编号：1000-2006（2025）06-0247-08

Abstract：【Objective】TheCcdC protein in Bacillus velezensis FZB42contains a DUF1453 domain withanunknown structureandfunction.Thisstudyaimstopredictthebiological propertiesofCcdCusing bioinformaticsmethodsandto investigateitsfunctionbyconstructingaccdC knockout strain，therebylayingthe groundwork forelucidatingthestructure andfunctionof theDUF1453domain.【Method】Bioinformaticsonline toolswereemployed topredicttheaminoacid composition，physicochemical properties，hydrophobicity，transmembraneregions，and subcellarlocalizationof the CcdC protein.A phylogenetic tree analysis of CcdC proteins from diferent bacterial species was also conducted.A ccdC knockoutstrainofB.velezensisFZB42wasconstructed，andthe growthof thewild-typeFZB42 strainand theccdC knockoutstrain wascompared inbothliquidand solidLBmedia.Additionally，spore formationofboth strains was assessed in DSM medium.【Result】The CcdC protein consisted of 16O amino acids and was a basic，stable，hydrophobic transmembraneproteinlocated inthecellmembrane，lacking asignal peptide.Secondarystructureanalysis revealed that the CcdC protein was predominantly α -helical.Tertiarystructure analysis indicated thatno structurally similar proteins to CcdC existed in current databases.Colony PCR confirmed the successul constructionof the ccdC knockout strain. Comparative analysis of growth andspore formation between the wild-type FZB42and the cdC knockout strain demonstratedthatthedeletionof theccdC gene significantlyafected bacterial growthand spore formation.【Conclusion] Bioinformaticsanalysis tentativelyidentified CcdCasamembrane protein，withpredicted phosphorylationsites suggestig itspotential involvement in signal transductionfunctions.Interaction network analysis indicated that CcdC proteinmayberelatedto growthanddevelopment.Theconstructionof the knockout strainconfirmed thatthedeletionof the cdCgene impair bacterialgrowthanddevelopment，providinga foundation for furtherresearch into the biological function of the DUF1453 domainand the regulatory role of CcdC in the growthand development of B . velezensis FZB42.

Keywords：DUF1453 domain; CcdC protein；bioinformatics analysis；growth and development；Bacillus velezensis

未知功能结构域（domian of unkown function，DUFs）是Pfam数据库中没有明确功能的结构域的统称[1-2]。（剩余15517字）