基于GSH-CuNCs的环境残留氧氟沙星快速检测法

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关键词：氧氟沙星（OFL）残留；铜纳米簇（CuNCs）;比率型荧光探针;氟喹诺酮类抗生素（FQs）；一锅法;快速检测 中图分类号：X822；S719 文献标志码： ΔA 文章编号：1000-2006（2025）06-0225-06

Abstract：【Objective】Thisstudyaims todevelopasimple，rapidandsensitivefluorescence detectionmethod for residual ofloxacin（OFL）in liquid samples，broadenthe applications of copper nanoclusters and providing atheoretical foundation for portable diagnostic devices.【Method】Aone-pot synthesis method was employed toprepareglutathionestabilized copper nanoclusters（GSH-CuNCs）with excellent watersolubilityand optical properties.Inanacidic condition，GSH-CuNCsand OFL have maximum emision peaksat425nmand 510nm respectively upon excitation wavelength at 340nm.With OFL，the green fluorescence intensityof thesystemat 510 nm was significantlyenhanced compared to GSH-CuNCs，meanwhile the blue fluorescence intensityat 425nm was slightlydecreased.Thus，a GSHCuNCs-basedratiometric fluorescentprobewasconstructed forthesimplerapid，sensitiveand selective detectionof residualOFLintheenviroment.【Result】Afteroptimizationoftheexperimental conditions，theratiometricfluorescent probe exhibited a linear response to OFL concentrationsin the range of 0.005-10.000μmol/L ，withadetection limit （LOD）of1.5nmol/L.Thisratiometricprobe has beenapplied to detect OFL in the actual spiked waterand milk samples.Applying with spiked real sample，the recovery rate of OFL was 93.6%-103.3% ，and relative standard deviations（RSD）were all less than 10% .【Conclusion】The developed ratiometric fluorescent probe offersthe advantages of simplicity，lowcost，high sensitivity，and excellnt selectivity，enabling rapidandaccurate detectionof residual ofloxacinin real-world applications.

Keywords：ofloxacin（OFL）residues；copper nanoclusters（CuNCs）；ratiometric fluorescent probe；fluoroquinolones （FQs）；one-pot synthesis method；rapid detection

氧氟沙星（OFL）是一种人工合成的氟喹诺酮类（FQs）抗菌药物，具有广谱、抗菌能力强以及与其他抗菌药物无交叉耐药性等特点，被广泛应用在临床医疗和畜牧养殖业[1-2]。（剩余12310字）