小果白刺bHLH基因家族鉴定与表达分析

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中图分类号：S793.9 文献标志码：A 文章编号：1000-2006（2025）06-0102-13

Abstract：【Objective】Thebasichelix-loop-helix（bHLH）transcriptionfactorsplaycriticalrolesinplant growth， metabolism，andresponses tobiotic/abioticstresses.Genome-wide identificationand bioinformaticsanalysisof the bHLH genefamilyinNitrariasibiricawillprovidefoundationalinsightsforsubsequentbHLHgenecloningandfurtherbiological studies.【Method】Bioinformatics and molecular biologyapproaches were employed tosystematicallyanalyze the phylogenetic relationships of the N. sibirica bHLH gene family at the genome-wide level. Transcriptome datasets and quantitativereverse transcription-PCR（qRT-PCR）were employed to analyze theresponseof thebHLH genesunder to stress.【Result】A total of 88 bHLH transcription factors were identified from the N ，sibirica genome，unevenly distributedacross 12chromosomes with two pairsof tandemly duplicated gene.Phylogeneticanalysis clasified these members intol6distinct subclades.Cis-acting element analysis revealed that the88 bHLH genes harbored multiple environment-and stress-responsiveregulatoryelements.Expresion profilingdemonstrateddiffrential responsesof N sibirica bHLH genes to salt stressqRT-PCR results further demonstrated that six bHLH genes （bHLH1，bHLH2， bHLH24，bHLH30，bHLH70 and bHLH73）exhibited pronounced tissue-specific expresson patterms.Under salt stress， bHLH1，bHLH24，bHLH70and bHLH73 were significantlyupregulated in leaves，while bHLH2 and bHLH3O were down regulated in leaves.Inrots，allsixgenesdisplayed marked upregulation.In stems，fivegenes（bHLH1，bHLH24， bHLH3O，bHLH7O and bHLH73）were upregulated，whereas bHLH2 showed down regulation.【Conclusion】This study identified88conservedbHLH transcription factors inN.sibirica，characterizedbyconserved genestructures and protein domains，along withtwo tandem duplication events onchromosomes.The diferential expresson of bHLH genes inroots， stemsand leavesunder salt stresssuggests their potential involvement insalt tolerance mechanisms.These findings establish acritical foundation for elucidating the functional rolesof the bHLH transcription factor family in N. sibirica.

Keywords：Nitraria sibirica；bHLH transcription factors；salt stress；gene expression

转录因子（transcriptionfactor）是真核生物中广泛存在的一类功能蛋白质分子，通过与DNA顺式作用元件结合调控基因表达，参与生物体生长发育及环境胁迫响应等过程[1]。（剩余21219字）