枸杞多糖缓解铝致Sertoli细胞毒性的机制研究

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中图分类号：R994.6 文献标志码：A DOI：10.3969/j.issn.1003-1383.2025.11.003

【Abstract】ObjectiveToinvestigate thetoxicefectsof aluminum exposureonSertolicells（TM4celline）andits underlying mechanisms，and to evaluate thealleviating effectof lycium barbarum polysaccharides（LBP）．MethodsTM4cells were divided into four groups：normal control （NC）group，aluminum exposure （Al）group，LBP control（NC + LBP）group， and LBP intervention （Al + LBP）group，and an aluminum toxicity model was established. Cell viability was detected by CCK8assay，apoptosisratebyflowcytometry，reactive oxygen species（ROS）levels byfluorescence microscopy，andtheprotein expressionsof glialcelline-derived neurotrophicfactor（GDNF），Catalase，superoxidedismutase2（SOD2），Bcl-2，BAX， Cleaved Caspase-9，and Cleaved Caspase-3 by Western blot.ResultsCompared with theNC group，theAl group showeda decreaseincellviability，anincrease inapoptosisrate，anincreaseinROSlevelsandthe protein expresionsofBAX，Cleaved Caspase-9，and Cleaved Caspase-3，and a decrease in the protein expresion of GDNF，Catalase，SOD2，andBcl-2（all P< 0.05）.Compared with the Al group，the Al + LBP group showed an increase in cell viability，a decrease in apoptosis rate，a decreasein ROSlevelsandpro-apoptotic proteinexpresion，andanincrease inGDNFandantioxidant/anti apoptoticprotein expression （all P<0.05 ）. ConclusionAluminum exposure damages Sertoli cells by inducing oxidative stress，downregulating GDNF expression，inibitingcellviability，andpromotingapoptosis.Conversely，LBPcanalleviatealumnuminducedSrtoli celltoxicitybyexerting antioxidantandanti-apoptotic efects，while maintaining GDNFexpressionandcellularviability.

【Keywords】aluminum exposure； Sertoli cells；reactive oxygen；apoptosis； lycium barbarum polysaccharides （LBP）

铝对雄性生殖系统的毒性作用已被证实，既往的研究主要集中在铝元素负面影响生精细胞及精子质量方面，而铝对睾丸支持细胞（Sertoli细胞）的具体毒性机制尚未明确。（剩余11039字）