网络药理学对蒲公英萜醇治疗结直肠癌的多维度调控机制研究

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中图分类号：R735.3 文献标志码：A DOI：10.3969/j.issn.1003-1383.2025.11.002

Study on multi-dimensional regulatory mechanism of network pharmacology ondandelion terpenoids in the treatment of colorectal cancer

LONG Pengchen’，LIU Tong'，WANG Yuncheng'，SU Di2a （204号 （1.DepartmentofGastrointestinalSurgery，AfliatedSouthuestHospitalofYoujiangMedicalUniversityforNationalitsBaise People's Hospital，Baise5330，Guangxi，China;2.DepartmentofGastrointestinal Surgery，Afliated Tumor Hospitalof Guangxi Medical University·Guangxi Clinical Research Centerfor Colorectal Cancer，Nanning 53O21，Guangxi，China）

【Abstract】ObjectiveToidentifythetargetsof taraxerol（TAX）inthe treatmentofcolorectalcancer（CRC），so as to provide theoreticalbasis fornewtreatment strategies.MethodsPharmMapperand SwissTargetPrediction wereusedtopredicthetherapeutictargets ofdandelion terpenoids，and standardization wasperformed by UniProt.TheGSE83129 dataset was downloaded from the GEO database，and limma package was used to scree the diferentially expressed genes （DEGs）. The therapeutic targetof TAXwas intersectedwiththe DEGs by Venny，and the targetofTAXforthe treatmentof CRC was obtained.And the protein-protein interaction network（PPI network）was constructed through STRINGand Cytoscape to scren the hub genes.The KEGG signaling pathways of hub genes were identified through GO/KEGG enrichment analysis， and the structure of the hub genes wasobtained from PDB，and the molecular docking test with TAX was conducted by AutoDock Tools.Finally，thedockingresultswerevisualizedthrough ΔPyMOL .ResultsThrough thePPInetwork，32hub genes were identified，including HSP90AB1， HSP90AA1，and CDK2，etc.Signaling pathways such as PI3K-Akt，p53， nod-likereceptors（NLRS），andinterleukin-17（IL-17）mightbeinvolvedinthe pathogenesis ofcolorectalcancer（CRC）. ConclusionTAX can target the HSP90AB1，HSP90AA1，CDK2 genes，as wellas the PI3K-Akt sigaling pathway，p53 signaling pathway，NOD-likereceptorsignalingpathwayandIL-17signalingpathwaytotreatcolorectalcancer，which indicatesthat TAXcanbeusedasa potential drugsfor the treatment of CRC，laying atheoreticalfoundationfor thesubsequent verification of therapeutic effects.

【Keywords】colorectal cancer（CRC）; taraxerol （TAX）； network pharmacology；hub genes； signaling pathways

结直肠癌（colorectalcancer，CRC）作为常见的侵袭性胃肠道肿瘤，具有确诊时多为中晚期、五年生存率较低等临床特征[1]，其恶性转化涉及TGF- ⋅β/ Smad、Hippo/YAP、EGFR/RAS/RAF/MAPK、PI3K/Akt-mTOR等多通路协同驱动，构成靶向治疗的重要分子基础[2-3];中医药干预在延缓肿瘤进程、增效减毒等方面展现独特优势[4]。（剩余12477字）