中国特有植物吴兴铁线莲组织培养再生体系的建立

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中图分类号：S567.23 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2025.06.005

Establishment of Tissue CultureandRegenerationSystemof Clematis huchouensis Tamura，an Endemic Plantin China

SHI Jianl，QIU Guoqiang'，WU Yanfen'，WANG Huifeng²，LI Jian²，ZHANG Feng³

（1.DeqngCountyEcologicalForsetryComprehensiveCenter，Huzhou，Zejiang332o，China;2.HangzhouHongsenForstyurey PlaningandDesign CompanyLimited，Hangzhou，Zhejang310o，China;3.ZhejiangForestResourcesMonitoring，Hangzou， Zhejiang 310000， China）

Abstract：Inordertorealizeindustrialproductionandaceleratetheopularizationandapplicationprocess，itisthebstwayto establish the plant tissue culture system of Clematis huchouensis.In this study，two-year old stem segments of clematis from Xing Wu with buds wereusedas explants fortissue cultureandrapid propagation.Theresults showed thatthe mixedtreatmentof 70% （20号 alcohol sterilization for 1O sand 10% sodium hypochlorite sterilization for1O min was the best，with a germination rate of 70.67% and a pollution rate of 17.33% .1/2MS medium is more suitable for start-up culture，and the sprouts grow well，not only robust，but also without yellowing and browning.In the multiplication culture， MS+2mg⋅L-16-BA+0.3mg⋅L-1 IBA +2 ， L-1 hydrolyzed casein was the best，and the multiplication coeffcient was 4.73.The best roting culture medium was 1/2 ms+0.5 mg·L -1 IBA+0.01 mg·L-1 NAA，and the rooting rate was 79.67% .This study established a complete tissue culture technology system for Clematis huchouensis， breaking itsgegapicalitatios.Itprovidesathoreticalbasisforitspromotionandommecialdvelopent，ndlsoerse tain support for its interdisciplinary cooperation in the later stage.

KeyWords：Clematis huchouensis Tamura; sterilization; tissue culture;culture medium

铁线莲属（ClematisL.）属于毛茛科（Ranunculaceae）植物，具有较高的观赏和药用价值-2。（剩余6895字）