基于KASP技术的SNP标记用于3个松花菜新品种种子纯度检测

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中图分类号：S641.3 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2025.06.001

ApplicationofSNPMarker BasedonKASPTechnologyin SeedPurityofThreeNewVarietiesofLoose-curd Cauliflower

LIU Lili'， JIANG Hanmin1， ZHANG Wei²，SHANXiaozheng1，WEN Zhenghual，ZHANG Xiaoli1 （1.StateKeyaboratoryofVegeablebreeding，ianjincadeyofAgiculturaliene，anjin3O92，a;2.ajKr nelAgricultural Scienceand TechnologyCompanyLimited VegetableResearch Institute，Tianjin3Ool92，China）

Abstract：Toadresstheintensecompetitionintheseedmarketandensuretherobustdevelopmentofthecauliflowerseedindustry genotyping technologywasemployedforpreciseandeficientvarietyidentificationandpurityassessmentofovellosecauliflower cultivars.Singlenucleotidepolymorphisms（SNPs）weredetectedusingthecompetitivealelespecific KompetitiveAlele Specific PCR （KASPPCR）techniqueonloosecauliflowerastheexperimentalmaterial.High-throughputsequencingfacilitatedteidentificationofNPlocisuitableforbrocolivarietydiscriminationthroughbioinformaticsanalysisSubsequently，thepurityofthenew SonghuavarietiesJnsong75，Jinsong90，andJinsong105waaseedusingtheidentifiedSNPlociandKASPgenotypingtecholo gy.Theresultsdemonstratedahighlevelofagreementbetwenthegenotypingresultsndpurityasessment（r0.904）.Inconclusion，theSNPmarker-basedapproachestablisedinthissudyofersareliablemethodforvarietyidenificatonandpurityassessment，therebyitigatingconomiclosesfromsdentifidhyrds，inimiingpotentialdisputes，hancingidentificatioey andacuracy，providingsubstantialevidenceforvarietyprotectionandpresentingsignificantpracticalvalueintheralmofSongua hybrid dentification and variety safeguarding.

Keywords： loose-curd cauliflower; KASP;SNP;seed purity

近年来，松散型花椰菜即人们常说的松花菜，因其独特品种优势备受青睐。（剩余6864字）