秀丽四照花组培快繁体系建立

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中图分类号：S688.9Q945.52 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2025.06.002

Establishment of Rapid Multiplication System for Tissue Culture of Cornus hongkongensis subsp.elegans RUAN Shuming，LAI Shigan， ZHOU Xianzhen

（FujianForestryVocational&TechnicalCollege，Nanping，Fujian353OOO，China）

Abstract：InordertoestablisharapidpropagationsystemforCornusflorida，tisseculturemethodswereused.Thestudyinvolved screenigsterilizationmethdsforstemsegmentexplantsofCorusflordandinvestigatingtheefectsofdiferentconcentratinsof hormonesNAAandBAoallsiuction，uddfrentiatio，oungleafproliferationdotigTeresultsidiatedtste ilization with 0.1% HgCl2 for 12 minuteswas optimal.Callus induction was most effectivewith M IS+1.0mg⋅L-16-BA+0.2mg⋅L-1 NAA， reaching a 90% induction rate after 40 days.Bud differentiation from callus was best promoted by MS+2.O mg·L -1 6-BA+0.2 mg·L-1 NAA. Shoot proliferation from sterile young leaves was highest with MS+1.O mg·L-1 NAA+0.5 mg·L-16-BA.Root induction was best in MS+0.5 mg·L-1 IBA，however，the induced roots were prone to browning，necessitating timely subculture onto a rejuvenationmediumIncondusion，thisstudyprovidesatechnicalfoundtionforthelarge-scalepropagatinofthisspecies，whichisof great significance for its resource development and industrial application.

Keywords：Cornus hongkongensis subsp.elegans; tissue culture; stem segment; callus

山茱萸科（Cornaceae）四照花属（Dendrobenthamia）植物，其头状花序近似球形，拥有4片大型白色花瓣状苞片，成熟时果实肉质呈红色，使其成为备受推崇的观花观果树种，是具有较好经济效益的乡土树种，同时在森林生态系统中也发挥着重要的生态效益[2-3]，在园林绿化、果用和药用等方面都有良好的发展前景4。（剩余9354字）