改良QuEChERS/UPLC-MS/MS法检测参类中药材中杂环酰胺类杀菌剂及其代谢物残留

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中图分类号：S567：X132 文献标识号：A 文章编号：1001-4942（2025）08-0139-10

AbstractAn analytical method was developed for the determination of fluxapyroxad，fluopyram，fluopimomide，furametpyr，penthiopyrad，thifluzamide，furpylamin fluopicolide and the metabolin 2，6-dichlorobenzamide residues simultaneously in Ginseng（Ginseng Radix Et Rhizoma），Salviae Miltiorhizae Radix Et Rhizoma American ginseng （Panacis Quinquefoli Radix），Codonopsis Radix through improving pre-treatment process and ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） methods.Samples were extracted with acetonitrile.Salviae Miltilorrhizae Radix Et Rhizoma extracting solution per milliliter was cleaned up with 180mg （202 MgSO4 ， 30mg PSA（ethylenediamine-N-propylsilane） and 3 mg GCB （SpeedTestTM activited carbon），while the extracting solution of Ginseng，American ginseng and Codonopsis Radix were cleaned up with 20mg PSA.The purified extracts were detected by UPLC-MS/MS with Waters ACQUITY UPLC？ HSS T3 column in positive ionization mode with multiple reaction monitoring and scanning， and the methanol solution containing 0.1% formic acid and aqueous solution containing 0.1% formic acid were used as mobile phase B and A respectively for gradient elute.The results showed that the 8 target compounds showed good linearity in the range of 0.0001 to 0.1mg/L with R2 over O.999. The limits of detection and limits quantitation were in the range of 0.000 01～0.000 6mg/kg and 0.0002～0.0010mg/kg ，respectively. The recovery rate was 76% to 120% at three supplemental levels of O.01，0.1O and 1.00mg/kg with the relative standard coefficient as 0.5% to 15.7% . This method had the characteristics of fast and simple pre-treatment process，good sensitivity and high accuracy，so it could be used to detect the residue of the seven heterocyclic amide fungicides and one metabolin in Ginseng and three similar herbal medicines.

KeywordsQuEChERS； UPLC-MS/MS； Ginseng and three similar herbal medicines； Heterocyclic amide fungicides ； Pesticide residue

吡唑和噻唑酰胺类杀菌剂同属于杂环酰胺类杀菌剂，是一类相对新颖的农药品种，具有极强的抗菌活性，主要作用于细胞膜和细胞间的特异性蛋白而表现杀菌活性，与其他类杀菌剂无交互抗性，对霜霉病、疫病、晚疫病、猝倒病等常见卵菌纲病害具有杰出防效[1-2]。（剩余10426字）