低温胁迫对4种竹子生理生化特性的影响及其耐寒性综合评价

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中图分类号：S795 文献标识号：A 文章编号：1001-4942（2025）08-0042-09

AbstractThe experiment was conducted to compare the physiological changes in leaves of four bamboo species under low-temperature stress and assss their cold tolerance，in order to provide bases for introduction and cultivation of cold-resistant bamboos. Four temperature treatments （O，-5， -10 and -15‰ ）were set to study their effects on chlorophyll（Chl） content，relative conductivity（REC），antioxidant enzymes activities， soluble protein （SP）content，and malondialdehyde（MDA）content in leaves from the upper，middle and lower parts of four bamboo species （Phylostachys bambusoides ，Phyllostachys nigra，Bambusa ventricosa and Pleioblastus amarus）.The results showed that as temperature decreased，the REC of leaves from different parts of the four bamboo species generally increased，the Chl content first rose then either fellor stabilized，the peroxidase（POD）and catalase（CAT） activities displayed the increasing-decreasing-increasing trend，the superoxide dismutase （SOD）activity and SP content mostly showed the decreasing-increasing-decreasing pattern，and the MDA content initially decreased then increased.Thecold tolerance of leaves varied across difrent parts of the plant.The upper leaves of Phylostachys bambusoides，lower leaves of Phylostachys nigra，and middleleaves of Bambusa ventricosa and Pleioblastus amarus had less cold tolerance compared with the other parts.Principal component analysis revealed that Chl content，SP content and POD activity had larger load values indicating significant efects on physiological changes in bamboos under low-temperature stress.Using membership function method，seven physiological and biochemical indexes of the four bamboo species were selected to comprehensively evaluate their cold tolerance，and the results indicated that their cold tolerance showed Phylostachys bambusoides>Phyllostachys nigra>Bambusa ventricosa>Pleioblastus amarus. This conclusion could provide the theoretical basis for scientific cultivationand frost protectionof leaves from different parts of these four bamboo species.

KeywordsBamboo； Low-temperature stress; Cold tolerance； Comprehensive evaluation； Physiologicaland biochemical characteristics

竹为禾本科竹亚科竹属多年生高度木质化的草本植物。（剩余11108字）