镁与多巴胺复合物提升杜梨幼苗耐盐碱性研究

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中图分类号：S661.2 文献标识码：A 文章编号：1002-2910（2025）05-0016-（

Binary ligand dopamine enhances saline-alkali tolerance in Pyrus betulaefolia seedlings

XU Huazhen1，BAI Haixiu²，FENG Shouqianl*

[1.College ofHorticultureScience and Engineering，Shandong Agricultural University，Tai'an，handong 271018，China；2.Shandong Medical Technician College，Tai'an， Shandong ， China）

Abstract： As an important plant growth regulator， dopamine can effectively mitigate the damage caused by saline-alkali stress. However， dopamine tends to self-polymerize in aqueous solutions to form polydopamine， thereby compromising its stress-alleviating capacity. When Mg2+ combines with dopamine to form a magnesium complex， it can effectively inhibit the self polymerization reaction of dopamine. To investigate the characteristics of magnesium complex and its alleviating effect on saline-alkali stress in pear seedlings， this study conducted relevant research. The results showed that at room temperature， Mg2+ coordinated with dopamine to form Mg/DA 5 which could inhibit DA auto polymerization， and UV spectroscopy showed that Mg2+did not significantly alter the chemical structure of DA. Under saline-alkali stress， the leaves of Du pear seedlings treated with polydopamine showed significant chlorosis and growth inhibition. The K+ and Na+ levels in the leaves and roots decreased significantly， and the K+/Na+ ratio decreased significantly. However， the growth of pear seedlings treated with Mg/DA was good， close to the control of water， and the relief effect was significant; The decrease in K+ and the increase in Na+ in the leaves and roots of pear seedlings treated with Mg/DA were the smallest， and the K+/Na+ ratio was the closest to CK，which could maintain ion balance； The expression levels of PbHKT1， PbNHXl，and PbSOS1 genes in leaves and roots were significantly increased， suggesting that they alleviate saline-alkali stress by promoting Na+ efflux or compartmentalization.

Key words： Pyrus betulaefolia; saline-alkali stress; dopamine; self-polymerization; Mg/DA ;ion content; gene expression

盐碱是一种广泛分布的非生物胁迫物质，已成为全球作物生产的主要制约因素之一[1]。（剩余6401字）