衡阳市烟草靶斑病病原菌的分离鉴定

打开文本图片集

引用格式：，等.衡阳市烟草靶斑病病原菌的分离鉴定[J].湖南农业科学，2025（5）：63-66.DOI：10.16498/j.cnki.hnnykx.2025.005.012

中图分类号：S432.1；S435.72 文献标识码：A 文章编号：1006-060X（2025）05-0063-04

Abstract：ToidentifythepathogencausingtobaccotargetspotinHenyangCitythisstudyemployedthetissueseparationmethodto isolatethepathogenfromthetypicaldiseasedtobaccoleavesinthisrgion.Thepathogenicityof teisolatewasvalidatedaccodingto Koch's postulate.Teisolatewasthenidentifedbasedonthecolonymorphologymicroscopicfeaturesofhyphae，andISsunce. TheresultsshowedthattheisolateybapresentedthetypicalmorphologicalfeaturesofRizoctoniasoanianditproducednopore butsclerotia.Thehyphae wereiitiallycolorlessandbcamelightbrowntoyellowbrownatmaturitywithsepta.TheISsqunce similaritybetweentheisolateandR.solani AG-3（HQ241274.1）washigh.Theconstructedphylogenetictreeshowcasedthatyba sharedthesamecladewithR.solaniAG-3（HQ241274.1）.Therefore，thepathogencausingtobaccotargetspotwasidentifedR.solani AG-3.

Key words：target spot; Rhizoctonia solani AG-3; isolation and identification; Hengyang

烟草（NicotianatabacumL.）是原产于南美洲的茄科（Solanaceae）烟草属（Nicotiana）一年生或有限多年生草本植物[1]，现已成为世界范围内广泛栽培的重要经济作物，在我国南北地区均有种植，具有较高的经济价值和潜在的药用价值[2-3]。（剩余6739字）