水稻OsIAA10蛋白的表达及纯化

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引用格式：，等.水稻OsIAA10蛋白的表达及纯化[J].湖南农业科学，2025（5）：1-5.DOI：10.16498/j.cnki.hnnykx.2025.005.001

中图分类号：Q945.78 文献标识码：A 文章编号：1006-060X（2025）05-0001-05

Abstract：OsIAA10playsanimportantroleinthegrowthdevelopment，andstressresponsesofrice.Tisstudyaims toexplorethe biochemicalnatureofOsIAA10.WiththecDNAof wild-type'Zhonghua11'（ZH11）plantsasthetemplate，andthecodingsequence of OsIAA10wasamplified withtheforwardandreverseprimersof pGEX-6p-1-IAA10.Then，therecombinant plasmid pGEX-6p-1- IAA10 containing OsLAAlO was obtained through vector construction and transferred into BL21 cells.After IPTG-induced expression， purification，ndGEdetection，aingleproteinbandosistentwihtheexpectatioasbaed，nditwaidtobe OsIAA10.Theiductionconditosfortheexpresioof tisproteiweretenoptized.Tesultshowedtatereatmetwith0. mmol/L IPTG at 37% for12 h were the optimal conditions for inducing the expression of OsIAA10.The research results underpin the subsequent research on the function of OsIAA10.

Keywords：rice;IAA1O;vector construction;protein purification

水稻（OryzasativaL.）是我国主要的粮食作物，全国约有 60% 的人口以水稻为主食[。（剩余8191字）