猕猴桃根部源分散泛菌葡萄糖异构酶异源表达研究

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中图分类号：0561.3 文献标志码：A 文章编号：1008-1038（2025）04-0035-06

DOI：10.19590/j.cnki.1008-1038.2025.04.006

Study on Heterologous Expression of Glucose Isomerase from Pantoea dispersa of Kiwifruit Root Origin

ZHI Yong， XIA Bo* （College of Food Science and Technology， Hunan Agricultural University， Changsha 410o00， China）

Abstract：Toaddressthelimitations of traditionalhigh-fructose syrup production，such as lowcatalytic efficiencyof conventional glucose isomerases，this study focused on the heterologous expressionand optimization of a novel glucose isomerase， P d -xylA， derived from Pantoea dispersa isolated from kiwifruit roots. The P d. -xylA gene was cloned and expressed in Escherichia coli BL21 （DE3），folowed by systematic optimization of induction conditions including temperature and IPTG （isopropyl- β D -thiogalactopyranoside） concentration. The results demonstrated that the optimal induction conditions were and 0.1 mmol/L IPTG， under which P d？ -xylA achieved the highest soluble expressonlevel.This study provided theoretical support for the development of enzyme resources with enhanced catalytic activity and industrial applicability， thereby advancing the biotechnological productionof

high-fructose syrup.

Keywords： Pantoea dispersa； glucose isomerase； optimization of induction conditions； heterologous expression

在食品工业和医药领域，随着消费者对甜味产品需求的不断增长，甜味剂的应用越来越广泛。（剩余10169字）