20种前胡属叶绿体基因组密码子偏好性分析

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中图分类号： Q943.2 文献标志码：A 文章编号：1673-3851（2025）09-0664-12

Reference Format： ， ， ，etal. Analysis codon usage biasin 2O Peucedanum chloroplast genomes[J]. Journal Sci-Tech ，2O25，53（5） ：664-675.

Analysis codon usage bias in 20 Peucedanum chloroplast genomes

ZHOU Mengli ，LI Hong ，GUO Lei ，WANG Xia ，WANG Ruihong （Collge ， Sci-Tech ， 31ool8，China）

Abstract：To investigate codon usage bias in 2O Peucedanum chloroplast genomes，we employed bioinformatics tools EMBOSS，CodonW， CUSP for codon composition analysis， visualized by SPSS2O. O. The study showed that the average GC base content was 39.89% ， GC3 content ranged from 30.02% to 30.22% ENC values ranged from 49.73 to 49. 90，indicating that the codon usage bias was weak；the sequences protein coding regions showed A/T base enrichment phenomenon， with a majority the third codons ending with A/U， neutrality plot other analyses showed that the main driver codon usage bias was natural selection. Twenty-nine high-frequency codons were screened， which 17 were the same optimal codons such as UUA，UGU UCU. Based on the extremely high similarity between the coding sequences the chloroplast genome the phylogenetic tree constructed from the whole chloroplast genome，it was shown that the codon usage bias was related to the evolutionary relationship. The findings this study lay a foundation for further research on molecular phylogenetics， genetic transformation， chloroplast genetic engineering in Peucedanum species.

Key words： Peucedanum ;chloroplast genomes；codon usage bias; phylogenomics； natural selection

0 引言

前胡属（PeucedanumL.）是伞形科（Apiaceae）中一个广泛分布且种类繁多的属，有 100～120 种，分布于欧亚大陆、南非和澳大利亚[1-2]。（剩余11697字）