基于转录组学的拟南芥AtNDX下游潜在靶基因挖掘与分析

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中图分类号：Q756 文献标志码：A 文章编号：1673-3851（2025）09-0646-10

Reference Format：WANG Jieyao，MA Rong， XING Kongya，et al. Mining and analysis of potential target genes downstreamof AtNDX in Arabidopsis thaliana based ontranscriptomicsJ]. Journal of Zhejiang Sci-Tech University，2025， 53（5）：646-655.

Mining and analysis of potential target genes downstream of AtNDX in Arabidopsis thaliana based on transcriptomics

WANG Jieyao ，MA Rong ，XING Kongya ，KE Liping，HUA Xuejun （Collge of Life Sciences and Medicine， Zhejiang Sci-Tech University， Hangzhou 31ool8，China）

Abstract： Plant mitochondria produce retrograde signals under environmental stress to regulate nuclear gene expresion and coordinate the balance between growth and stress resistance， yet the underlying mechanisms remain poorly understood. First，we performed transcriptome sequencing on roots of Arabidopsis SSR1 mutants （ssrl-2）， ssrl-2 suppressor （sus3）， sus3 complemented line （sus3C），and Wasilewskija （WS） wild-type plants. Second， principal component analysis （PCA） and Pearson correlation coefficients were used to assess the corelations among transcriptomic data， followed by generating volcano plots and cluster heatmaps of differentially expressed genes （DEGs）. Finally，by integrating KEGG and GO analyses，genes simultaneously regulated by mitochondrial stress and AtNDX were screened to elucidate the mechanistic role of AtNDX in modulating plant growth and development under mitochondrial stress. The results showed that the high-throughput sequencing data of the four materials exhibited good reproducibility，with ssrl-2 and sus3C displaying a high correlation，and sus3C was able to complement the effects caused by the suppressor mutation in sus3.A total of 288 genes were identified as being negatively regulated by AtNDX under mitochondrial stress，while 178 genes were positively regulated. Quantitative real-time PCR（qRT-PCR） validated 15 candidate downstream target genes of AtNDX ，all of which play critical roles in plant growth and cellular metabolism. Our findings demonstrate that AtNDX modulates mitochondrial retrograde signaling and plant development by regulating root development and metabolic pathways， providing key insights into how plant mitochondria balance growth and stress adaptation.

Key words： Arabidopsis thaliana ; transcriptome; AtNDX；downstream target gene; mitochondrial stress

0引言

线粒体是细胞的“能量工厂"和代谢中心，在植物响应体内生长发育信号和体外环境因子刺激的平衡中起着非常重要的调控作用[1-3]。（剩余12722字）