基于网络药理学和体外实验拓扑分析三七总皂苷治疗痛风性关节炎的作用机制

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中图分类号：R288 文献标志码：A 文章编号：1002-4026（2025）05-0040-09

Abstract：Inthis study，weanalyzed theactive componentsand mechanismof Panax notoginseng saponin（PNS）in treatment of goutyarthritis（GA）through network pharmacology，moleculardocking，andcelular experiments.An in vitro hyperuriemia cellmodelwasestablished using adenosineand xanthine oxidase toiduce HK-2cells.Moreover，theuric acid-lowering activityofPNSat diferent doses wasassessed.Network pharmacologywasused toexplore the potential mechanism of PNS inthetreatment of GA，and keytargets were validated using moleculardocking.Results revealed that PNS couldinhibit thereleaseof uricacidfromrenaltubularepithelialcels that was inducedbyadenosineand xanthine Oxidase.Inaddition，15 key targets relatedto GA intervention were identiedfromPNS.Resultsof Gene Ontologyand Kyoto Encyclopedia of Genesand Genomesanalysessuggested that PNS may exert therapeutic effects onGA by regulating multiple signaling pathways，such as JAK2-STAT3，AGE-RAGE，and calcium signaling.The molecular docking results showed that the binding energies of each activecomponent with keytargets such as STAT3，PTAFR，and IL2 were all lower than ， indicating good afinity，which can be used as potential therapeutic targets.This study provides areference for the use of PNS in the treatment of GA.

Keywords ： Panax notoginseng saponin；gouty arthritis；network pharmacology；molecular docking；mechanism

痛风性关节炎（gouty arthritis，GA）是一种由尿酸代谢紊乱引发的最常见的关节炎症性疾病，或成为仅次于糖尿病的第二大类代谢性疾病[。（剩余13348字）