联合指纹图谱和活性综合评价的荆防颗粒质量控制研究

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中图分类号：R285 文献标志码：A 文章编号：1002-4026（2025）05-0022-09

Abstract：Combined fingerprinting and comprehensive assessment of activity techniques were used in this study to determine thequaity control of Jingfang granules.Separation wasperformed usingan Agilent TC-C18 chromatographic column （ 4.6mm×250mm ， 5μm ），gradient elution performed using an acetonitrile- .0.8% formic acid aqueous solution，and ESI-TOF/mass spectrometer was used for analysis.Thechemical constituents of Jingfang granules wereidentified through the precise molecular weightinformationof the parent anddaughter ionsof compoundsandby consulting references. Moleculardocking wasusedtoexamine thebondingabilityof the following fivechemical componentstoneuraminidase （NA）： prim-O-glucosylcimifugin（POG），ferulicacid，hesperidin，naringin，and sec-O-glucosylhamaudol（SOGH）.The antioxidant activityof thefivecomponentswasevaluatedbyanABTSfreeradical scavenginginvitroexperiment. Fingerprintingandquantitative assaywere used toevaluatethequaityof Jingfang granules.Fromtheresults，twenty-four chemicalcomponents wereidentified within the Jingfanggranules.Molecular dockingresultsrevealed the high binding capacity of NA to POG，ferulic acid，naringin，and SOGH，with a minimum recorded binding energy of .Free radicalscavenging invitroexperiments revealedthatal thefivecomponents exhibitedcertainantioxidantactities.The compositional similarityof diferent batches of Jingfang granuleswas foundtoberelativelyclose，but there were some diferenes inthecontentof thefivecomponents，whichmaybeatributedtodiferences inthesources of Chineseherbal medicines.This study provides methodological andconceptual supportforoverallqualityevaluationstudies of Jingfang granules.

Keywords ： Jingfang granules;fingerprinting；comprehensive assessment of activity；molecular docking

流感具有传染性强、传播快、易发生变异等特点，对人类健康产生严重影响[1-2]。（剩余10267字）