响应面法优化酶-水连续提取西洋参须根人参皂苷

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中图分类号：R284.2 文献标志码：A 文章编号：1002-4026（2025）05-0010-12

Abstract：To optimize theextraction process for ginsenosides from the fibrous rots of American ginseng，the enzymewater methodwas usedforcontinuousextractionof ginsenosides from American ginseng.Thetotal ginsenosides extraction yield was usedas the evaluation index，andtheextraction process wasoptimized using theresponse surface methodology.The results showed that the optimal enzymatic extraction conditions were as follows： ∝ -amylasewithan enzyme concentration of 7.3% ，a liquid/solid ratio of 30mL/g ，and an enzymatic hydrolysis time of 1.2h . After enzymatic hydrolysis，a secondary water extraction was performed，andtheoptimal condions wereas folows：aliquid/solidratioof 27mLg ，an extraction temperature of 89∘C ，and an extraction time of 1.3h . Under these conditions，the total ginsenosides extraction yield reached 16.61% . Since it is stable，simple，and eficient，this method has significantly improved the extractionyieldandavoidedthewasteof resources.Itpartiallfilsagapintheextraction processof total ginsenosides fromthefibrous roots ofAmerican ginsengand providesareferenceforfurtherpurification，refinement，andactivitystudies of total ginsenosides.

Keywords ：American ginseng；fibrous roots；ginsenosides；extraction process；response surface methodology；single factor experiment；ultraviolet spectrophotometry

西洋参（Panax quinquefolium L.）又名花旗参、洋参或西洋人参[1]，是五加科人参属多年生草本植物，可作药食两用。（剩余11767字）