支气管败血波氏杆菌前噬菌体特征分析

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中图分类号：R446.5 文献标识码：A 文章编号：1001-8751（2025）04-0234-10

AnalysisoftheProphage CharacterisationbyBordetella bronchiseptica

Wang Chuan-xu1，YueDong-xue1， Sun Ye-hui1，Cui An-qi1， ZhouLi-sheng1， Liu Wen-hual， Xu Shou-zhen1， Zou Ling1，Ma Jing1， Qu Xue-ting²， Yin Yan-bo²， Zhang Can1 （1 Qingdao Agricultural University，Qingdao 2660oo；2 Qingdao Bolong LaboratoryAnimalCo，Ltd，Qingdao26000）

Abstract： ObjectiveThis study aimed to analyze the prophage in101 strains of Bordetella bronchiseptica（Bb）. MethodsMolecular typing of each Bb strain was carredoutusing theMLSTtool.The prophages in Bb strains were analyzed by the PHASTEST tool. The resistance genes and virulence genes Were analyzed in the CARD or VFDB database.Genomic characterization and evolutionary analysis were conducted for identified prophages.Results The 101 Bb strains were categorized into 37 sequence types （STs）and primarily originate from humans，pigs，and horses in the US， China，Denmark，and Hungary. The genomic length of Bb was concentrated in 4.6～6.0Mb ，and the GC content was 67.5%～69.0% . All of Bb strains carried prophages.A total of 270 prophages were detected， 51 of which were intact prophages. The proportion of prophages in the Bb genome was only 0.1067%～1.069% . The analysis of resistance genes and virulence genes showed that 3O strains of Bb carried9 types of resistance genes，mainly sulfonamide and tetracycline resistance genes.There were two prophages that carried the virulence factor of the type III secretion system （T3SS）.ConclusionProphages，which donotcontribute to the horizontal transferofresistance genes inBb，are frequentlycarriedbyBband may increase the pathogenicityof theBbby making the hostbacterium more virulent.

Key Words： Bordetell bronchiseptica;prophage; drug resistance gene; virulence gene; molecular type; feature analysis

支气管败血波氏杆菌（Bordetellabronchiseptica，Bb）是一种具有多形态的革兰阴性杆菌，属于β -变形菌纲（Betaproteobacteria）中的波氏杆菌属（Bordetella）。（剩余15171字）