LRP5影响胶质母细胞瘤细胞增殖及MAPK信号通路的研究

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【中图分类号】 R739.41 【文献标志码】 A 【文章编号】 1672-7770（2025）04-0393-07

Study on effects LRP5 on glioblastoma cell proliferation andMAPK signal pathways LIYongshi， JIN Xin，LI Huibing，LI Guojun，LIU Pengfei，KANG Zhengwen，FU Yulin，DENGShifeng. ， ChinaCorresponding author ： JIN Xin

Abstract： Objective To analyze the expression LRP5 in glioblastoma（GBM） and explore the effect knocking down LRP5 on cell proliferation，migration abilities and MAPK signaling pathway. MethodsThe GEPIA database was used to analyze the diference in the expression LRP5 between GBM and adjacent tissues. The cancer and adjacent tissues and sections patients diagnosed bypathologywere collected，and the bioinformaticsresults were verifiedby immunohistochemistry and Western Blot. Small interfering RNA（siRNA） technology was used to knock down LRP5 for cell function experiments to observe the effects LRP5 on the proliferation， migration ability and MAPK signaling pathway GBM cell lines. ResultsBioinformatics analysis found that the expression LRP5 was increased in GBM tissues and was positively correlated with Ki-67.The results clinical tissue sample verification were consistent with the results bioinformatics analysis. The results cell experiments showed that knocking down LRP5 significantly inhibited the proliferation and migration ability GBM A172 cell line，increased the proportion cells in the G2/M phase，and inhibited the JNK and p38 signaling pathways.

ConclusionsLRP5 may promote the proliferation and migration GBM by affecting the cell cycle and activation the MAPK pathway， indicating its value as a potential molecular target for GBM.

Key words： glioblastoma；LRP5；proliferation；migration

胶质母细胞瘤（glioblastoma，GBM）是颅内常见的原发性恶性肿瘤之一，具有高度侵袭性的特点，通常发生在大脑半球，约占所有原发性中枢神经系统恶性肿瘤的一半[1]。（剩余10719字）