FMNLI对胶质瘤细胞增殖、迁移和侵袭的影响

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【中图分类号】 R739.41 【文献标志码】A 【文章编号】1672-7770（2025）02-0181-08

Abstract： Objective To investigate the effect FMNLl on the proliferation， migration and invasion glioma cells， and to provide a new potential target for the clinical treatment glioma patients. MethodsThe Cancer Genome Atlas（TCGA） and Chinese Glioma Genome Atlas （CGGA） databases were used to explore the relationship between FMNLl and glioma grade and prognosis patients. The effect FMNLl on the proliferation， migration and invasion glioma cells was investigated by CCK-8， cell scratch and Transwell migration and invasion function experiments. Western blot（WB） was used to detect the epithelial mesenchymal transition（EMT）in U251-shFMNLl， U251-OEFMNLl， U87-OEFMNLl and LN229-OEFMNLl cell lines Transition （EMT）， and to explore the effect FMNLl on EMT. Results The database clinical samples showed that the expression level FMNLl was positively correlated with the WHO grade glioma and significantly affected the survival time glioma patients. Knocking down or up-regulating the expression FMNLl had a significant effect on the proliferation， migration， and invasive ability glioma cells. The results WB showed that knocking down the expression FMNLl significantly elevated the expression E-cadherin expresson was significantly elevated， while the expression N-cadherin and Vimentin was significantly decreased after knocking down the expression FMNLI. After up-regulating the expression FMNLl， the expression E-cadherin was significantly decreased， while the expression N-cadherin and Vimentin was significantly increased. The expression level FMNLl is closely related to the WHO grade and the prognosis

glioma patients. As a cancer promoting factor， FMNLl can significantly improve the proliferation， migration and invasion ability glioma cell， and at the same time FMNLl promotes the EMT process glioma cells.

Key words： glioma； FMNLl ； proliferation； migration； invasion； EMT

胶质瘤是颅内最常见的原发性恶性脑肿瘤，大约占颅内肿瘤的  。（剩余11970字）