MTHFD2对脑胶质母细胞瘤细胞生长和转移的作用和机制研究

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【中图分类号】R739.41【文献标志码】A【文章编号】1672-7770（2025）02-0166-08

Abstract： Objective To explore the effects methylenetetrahydrolate dehydrogenase 2 （MTHFD2） on the growth and metastasis glioblastoma（GBM） cells and its mechanism. Methods The expression level MTHFD2 in GBM tissues and normal tissues was analyzed by interactive gene expression prile analysis database（GEPIA）. U87 MG cells were divided into control group， si-NC group and si-MTHFD2 group. The changes glycolysis and oxidative stress， as well as cell proliferation， apoptosis， migration and invasion were detected in each group. Xenotransplantation model nude mice was established and divided into sh-NC group and sh-MTHFD2 group. Tumor volume was measured and tumor growth curve was drawn. The expression levels MTHFD2 gene were detected by RT-qPCR， and the expression levels glycolysis key enzymes and epithelialmesenchymal transition marker proteins were detected by Western blot. Results GEPIA database analysis showed that MTHFD2 was highly expressed in GBM tissues （ ）. Compared with normal NHA cells， the relative expression levels MTHFD2 mRNA and protein in U87 MG cells were increased （ ）. Silence MTHFD2 inhibited the relative glucose intake and lactate production U87 MG cells， inhibited clone formation， cell proliferation activity， cell migration and invasion， and promoted the level oxidative stress and cell apoptosis.In the tumor formation experiment nude mice， silence MTHFD2 inhibited tumor growth. Conclusions MTHFD2 is a promoter malignant progression GBM. The silencing MTHFD2 expression can effctively inhibit cellular glycolysis stress， suppress cell proliferation， migration， invasion， and tumorigenesis in vivo， while also enhancing oxidative stress and apoptosis.

Key words： glioblastoma； methylenetetrahydrolate dehydrogenase 2； glycolysis； oxidativestress； proliferation and apoptosis； migration and invasion

胶质母细胞瘤（glioblastoma，GBM）起源于星形胶质细胞，是最具侵袭性和最常见的恶性原发性脑肿瘤，根据世界卫生组织分类，属于高级别恶性胶质瘤（IV级）[1]。（剩余11410字）