子午流注针法对失眠大鼠GIu/GABA-GIn代谢环路异常的调节作用

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Ziwuliuzhu acupuncture modulates Glu/GABA-Gln metabolic loop abnormalities in insomniac rats

XUJiarong²，HUANG Ao1，2 ，DINGZhikai²，BAOYul，ZHAOCnghuan，AIW

Departmentalli Uniersityo;tuedali China

Abstract：ObjectiveToinvestigatethetherapeuticefectofZiuuliuzhuacupunctureinaratmodelofinsomniaandits regulatoryeffecton theglutamicacid（Glu）， γ -aminobutyricacid（GABA）-glutamine （Gln） metabolic loop.MethodsFortymale SDratswererandomlyassignedtoontrolgroupmodelgroup，NajiagoupndNaigroup（n10）.Ithelatter3roat modelsof insomnia wereestablishedbyintraperitoneal injectionsof p-chlorophenylalanineandverifiedusingasodium pentobarbital-induced sleeptest.After modeling，theratsinNajiaandNazi groupsreceivedacupuncturefor7daysat specificallychosensetsofacupointsbasedontheZiwuliuzhurationaleintraditionalChinesemedicine.Pathologicalchangein the hypothalamic tisse of therats were examined withHEstaining，andthe levels of Gluand GABA in the hypothalamus were determined withhigh-performance liquid chromatography （HPLC）-mass spectrometry （MS）/MS.Immunohistochemistry was used to detectthe expressions of GABAA receptors （GABAARs）in the hypothalamus，and the expression levelsof glutamatedecarboxylase （GAD65/67）andglutamine synthetase （GS）were determinedwith Western bloting.Results Compared withthemodel group，therats inNajiaandNazi groups exhibited decreased Glulevelsand GABAAreceptor expresionand increased GABA levels with a decreased Glu/GABA ratio in thehypothalamus. Ziwuliuzhu acupuncture significantlyincreasedtheproteinexpresionsofGAD65andGAD67andloweredtheexpressionofGSinthehypothalamus intheratmodelsof insomnia.Conclusion Ziouliuzhuacupunctureproducessedativeandhypnoticefects inratmodelsof insomniaposiblybyregulatingGluand GABA-Glnmetabolism torestoretheexcitatory/inhibitorybalancebetween Gluand GABA.

Keywords： acupuncture; glutamic acid; γ -aminobutyric acid; glutamic acid/γ-aminobutyric acid-glutamine metabolism loop; insomnia

目前，失眠是世界上最严重的健康问题之一1。（剩余18574字）