孕期恐应激通过降低大鼠胎盘氨基酸转运体表达损害子代认知发育
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Prenatal fear stress impairs cognitive development in offspring rats by disrupting placental amino acid transport
DUZhixin1，WNGueyang，NGiping1，OJunlin，Jaua²，Aengbe，WNGaohui²，oln Schoolofoeidlictte Affilatedsaitaldedl SouthernedicaliversityGuangzhou515，in
Abstract： ObjectiveTo investigate the impact of prenatal fear stress on placental aminoacid transportandemotion and cognitiondevelopment inofspringrats.MethodsThirtypregnantWistarratswererandomizedequallyintocontroland fear stress（inducedusinganobservationalfootshockmodel）groups.Ineachgroup，placentalandserumsampleswerecollected from6damsongestationalday2O，andtheremainingratsdeliverednaturallyandtheofspringratswereraisedunderthe sameconditionsuntil8weeksofage.Emotionalandcognitiveoutcomesoftheoffspringrats wereasessed withbehavioral tests，andplacental structurewasexaminedusingHEstaining.Bioinformaticsanalysiswasused toidentifydiferentially expresed placental transporter genes under fear stressThe expresionsof system AandsystemLaminoacid transporters， alongwithotherspecializedtransporters，weredetectedusingqRT-PCRandWesternbloting.Fetalserumaminoacid concentrations weredeterminedbyHPLC.Thecorelationsbetweenfetalaminoacid levelsandbehavioraloutcomesof the ofspringratswereanalyzed.ResultsThedams withfearstressshowedreducedopen-fieldactivityandincreasedfreezing behaviorwithsignificantlyecreasedplacentalweight，fetalweight，andfetal-toplacentalratio.ioinformaticsaalyis revealed28diferentiallyexpressedtransportergenes involvedmainlyinaminoacidtransport.Inthefearstressgroupfetal serumaminocidlvelsresignificantlylweeddS38al，c4a1，43a，a8，6a6，lcaandSaad proteinexpressions werealldownregulated.Theofspringratsinfearstressgroupexhibiteddecreasednovelobjectpreference andspontaneousalternationwithreducedopenarmexplorationandincreasedimmobilityinemotional tests.Lowerearly-life aminoacidlevelswasfoundtocorrelatewithimpairedadultcognition.ConclusionPrenatalfearstressinratsimpairs placentalaminoacidtransporterexpressonandreducesfetalserumaminoacidlevels，potentiallycontributingtoo-term cognitive deficits in the offspring rats.
Keywords： fear stress; placenta;amino acid transport; offspring emotion; cognition development
全球约 15% 的孕产妇受不良情绪困扰，其中恐惧情绪的发生率可达 8%～14%[1] 。（剩余14613字）
