芪芎左归颗粒通过上调BDNF/TrkB通路提高衰老大鼠突触可塑性

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Qixiong Zuogui Granules enhance synaptic plasticity in aging rats by regulating the BDNF/TrkB signaling pathway

WANGQinggel，ZHAOXiaohui²，HEYuxuan2，IUeixiang3，ZHANGuke

1TalentPoolofennronceHospitalofadiioalineedicineengouin;sticaldicallf HenanUniesiteicnerttoeotsfedial Universityofineseedicine，Zhengzhou5oooo，ina

Abstract： Objective To exple the mechanism of Qixiong Zuogui Granules （QXZG） for enhancing synaptic plasticity in aging rats.MethodFortySDrats wererandomized intocontrol group，agingmodelgroup，donepezil treatment group，and QXZG treatmentgroup（n10）.Exceptforthcontroats，allteatsweresubjectedtodailyintraperitonealijectionofDgalatoe for8consecutive weeks toinduce brainaging，and donepezil hydrochlorideandQXZGsuspension were administeredby gavagedurigmodeling.Afterteterenions，teatswereevauatedforgenralonitions，eavioralngesidtive stressindicators，ppocaalpathologiesandexpressionsofthebaideivedurotroicactor（D）/tyoieise receptorB（rkB）pathwayp16andsyapticplasticity-associatedprotens.ResultsTeratsinthemodelgroupbied obviousagingphenotypessuchasyellowingof theteethandhair，bodyweightlossandimpairedlearingandmemory abilities，withdecreasedserumSODandGSH-PxactivitiesandincreasedserumMDAlevel.Theratmodelsalsoshowed obviouspathogcalgcdissiesndted16oteepsiineppoaloith significantlydecreased expressionlevelsof BDNF，TrkB，CREBandsynaptic plasticityproteins SYN，GAP43，andD95. Treatment with QXZG allviatedtheagingphenotypes intheratmodels，improved their learningand memoryabilitiesand pathologicalcanges intheppocapalCAegion，ducedoxdativestressandp16proteiexpresion，andprootedthe expresions of the BDNF/TrkB pathwayproteinsand synaptic plasticity proteins.Conclusion QXZG enhances synaptic plasticityand reduces oxidative stressin aging rats possibly by upregulating the BDNF/TrkB signaling pathway proteins， thereby delaying brain aging and improving learning and memory abilities of the rats.

Keywords： brain aging; BDNF/TrkB signaling pathway; synaptic plasticity; learning and memory; Qixiong Zuogui Granules

衰老又称老化，指随着时间的推移，生物体内环境稳态失调并对应激刺激等适应能力下降，出现组织器官结构改变和生理功能退化的现象[1]。（剩余15839字）