鸢尾素通过MEK1/RanBP2信号通路对心肌缺氧复氧损伤的保护作用

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[关键词］鸢尾素；心肌细胞；缺氧复氧损伤；急性心肌梗死

[中图分类号] R542.22［文献标志码]A [文章编号] 2097-5716（2025）05-0659-06

DOI： 10.13308/j.issn.2097-5716.2025.05.007

[引用格式]，，，等.鸢尾素通过MEK1/RanBP2信号通路对心肌缺氧复氧损伤的保护作用[J].实用心电与临床诊疗，2025，34（5）：659-664.

Protective effect of irisin against myocardial hypoxia/reoxygenation injury via MEK1/RanBP2 signaling pathwayWANG Yangyang，WANG Hao， YANG Jingwei，GU Yunfei（Department of Cardiology，Luoyang Central Hospital Affiliated to Zhengzhou University， Luoyang Henan 471OOo， China）

[Abstract]ObjectiveTo investigate whether irisin exerts its protective efect against myocardial hypoxia/ reoxygenation （H/R） injury by regulating the MEK1/RanBP2 signaling pathway.MethodsAn H/R injury model was established by using H9C2 cardiomyocytes. The H9C2 cells were randomly divided into four groups：a control group （untreated H9C2 cels），an H/R group （H9C2 cells subjected to H/R injury），an irisin group （H9C2 cells pretreated with 10ng/mL irisin for 2h ），and an H/R+ irisin group （H9C2 cells pretreated with 10ng/mL irisin for 2h followed by H/R injury）. Cell viability was assessed using the MTT assay，and cell death rate was evaluated by lactate dehydrogenase leakage rate. Mitochondrial dysfunction induced by H/R injury was also detected. Changes in the expresion of the E3 ubiquitin ligase（RanBP）2 andMEK1 signaling molecules were analyzed by immunoblotting.ResultsIrisin significantly inhibited H/R-induced H9C2 cardiomyocyte death.Thecellviability rate was only （43.28±2.37）% in the H/R group， however it increased to （76.24±6.28）% after irisin pretreatment （ P=0.000 7. ），and the cell death rate decreased from （57.38±4.38）% to （26.38±3.29）% （ P=0.000 4 ）. Additionaly，irisin effctively inhibited theopening of the mitochondrial permeability transition pore induced by H/R injury.Mechanistic studies further revealed that the protective effect of irisin was associated with theregulation of RanBP2 celllar localization via the MEK1 signaling pathway.ConclusionIrisin protects against myocardial H/R injury by inhibiting MEK1-mediated sequestration of RanBP2 and altering thecellular localization of RanBP2. [Key words]irisin； cardiomyocyte； hypoxia/reoxygenation injury；acute myocardial infarction

急性心肌梗死（acutemyocardial infarction，AMI）作为心血管领域的急危重症，在老年人群中患病率高达 9.5% ，对患者的生活质量及生命安全构成严重威胁[1]。（剩余9910字）