响应面优化兰州百合多糖大孔吸附树脂纯化工艺及其活性
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中图分类号：0625.3 文献标志码：A DOI：10.3969/j.issn.1003-9015.2025.00.013
Abstract：The processconditions for purifying crudepolysaccharides from Lanzhou lily were optimized using macroporous adsorption resin，andthe whitening，moisturizing，and hygroscopic activities ofboth crude and purified polysaccharides were evaluated. Through static experiments，DM3O1 was selected as the optimal resin.Based on dynamic experiments，the weighted comprehensive score （including decolorization rate，protein removal rate，and polysaccharide retention rate）was used as the response value to optimize the optimal processconditions for DM301 purification of lily polysaccharides using response surface methodology.The whitening activity was evaluated bythe inhibition of Polysaccharide on monophenolase and diphenolase.The moisture retention and hygroscopic activities of thesampleswere investigated at 22∘C and relative humidityof 11% ， 43% ， 81% and 95% within24hours.Under the conditions of a sample concentration of 4.30mg⋅mL-1 ，eluent volume of O.70 BV，flow rate of1.36 BV·h-1 ， and sample volume of 1.0BV ，the comprehensive score reaches 63.99%±0.02% （ n=3 ），which is close to the predicted value，indicating thattheestablished mathematical model is reasonable.The purified polysaccharides exhibited good whitening activity，which IC50 values for the inhibition rates of tyrosinase monophenolase and diphenolase are 3.52mg⋅mL-1 and 2.79mg⋅mL-1 ，respectively. At 22∘C and four humidity conditions，both crude polysaccharides and purified polysaccharides exhibit excellent moisture absorption properties （with a 24-hour moisture absorption rate greater than 100% ）.Purified polysaccharides and their mixtures have high moisturizing properties （24- hour moisturizing rate greater than 97.5% ）.The optimization of the purification process of crude lily polysaccharides using macroporous adsorption resin combined with response surface methodology is stable and feasible，providingatheoretical basis forthe efective purificationoflily polysaccharides.The whitening，moisturizing，and hygroscopic activities of purified lily polysaccharides have beenenhanced，providing experimental basis for the development of Lanzhou lily polysaccharides.
Key words：Lanzhou lily polysaccharide；macroporous adsorption resin；response surface methodology；whiteningactivity； hygroscopicity and moisture retention
1前言
百合（Lanzhou lily）拉丁名为 Lilium davidiivar．unicolor Salisb，为百合科（Liliaceae）百合属（Lilium）川百合的一个变种，味醇香甜，营养丰富，是唯一食药两用的甜百合，用于阴虚燥咳、劳嗽咳血、虚烦惊悸、失眠多梦等[1]。（剩余17741字）
