基于网络药理学和分子对接技术探讨菟丝子改善卵巢储备功能减退作用机制

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[中图分类号］R285 [文献标志码］A [文章编号]0256-7415（2025）13-0211-10

DOl：10.13457/j.cnki.jncm.2025.13.036

Exploring Mechanism of Cuscutae Semen in Improving Diminished Ovarian Reserve Based on Network Pharmacology and Molecular Docking Technology

ZHU Yue1，LIU Ziqin1，JIN Xuening1，CUI Shu'na1.2 1.MedicalColegeInstituteofTranslationalMedicine，YangzhouUniversity，YangzhouJiangsu225o09，China；2.Departmentof Obstetrics and Gynecology，Affiliated Hospital of Yangzhou University，Yangzhou Jiangsu 225oo9，China

Abstract：Objective：To analyze the mechanism of Cuscutae Semen in improving diminished ovarian reserve（DOR）using network pharmacology and molecular docking technology.Methods：The active components of Cuscutae Semen were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（TCMSP）.Potential targets of the activecomponentsand DOR-related targetswere predicted through GeneCards，OMIM，and DisGeNET databases.A protein-protein interaction network was constructed using the String databaseand visualized with Cytoscape software.GO and KEGG enrichment analyses of the intersecting targets were performed using the Hiplot database.Finally，moleculardocking verification was conducted using AutoDock software. Results：Cuscutae Semen contains 14 activecomponents including kaempferol，quercetin，and isorhamnetin，with 58 potential targets for DOR regulation，including 10 key targets：AKT1，EGFR，TNF，IL-6，TGFB1，CASP3， MMP9，BCL2，ESR1，and TP53.The core pathways mainly included lipid and atherosclerosis，PI3K-Akt signaling pathways，etc.Molecular docking results showed good binding activity between core componentsand key targets. Conclusion：The main active components of Cuscutae Semen （kaempferol，quercetin，isorhamnetin，etc.） exert reproductive protective effcts byactingon targetssuch as AKT1，EGFR，TNF，and IL-6 to activate or inhibit signaling pathways including lipid and atherosclerosis，PI3K-Akt，etc.

Keywords：Diminished ovarian reserve；Cuscutae Semen；Network pharmacology； Target prediction；Mechanism

卵巢储备功能减退（DOR）是指卵巢内卵母细胞数量和（或）质量下降，伴随着窦卵泡数（AFC）下降，促卵泡刺激素（FSH）升高，抗缪勒氏管激素（AMH）下降。（剩余10022字）