魔芋软腐病病原菌分离鉴定及室内药剂筛选

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中图分类号：S432.4 文献标识码：A文章编号：1673-9868（2025）10-0014-13

DOI：10.13718/j.cnki.xdzk. 2025.10.002

Abstract：Soft rot is a common disease in the production of konjac. The disease is very difficult to control after infection of plants，which can cause yield loss or even crop failure in large-scale konjac production. Isolation and identification of pathogenic bacteria causing konjac soft rot，and screen effective green compound pesticide combinations to provide strategies for green，efficient control of disease in konjac production. In this study，the pathogenic bacterial strains were isolated and purified from diseased konjac plants by tissue isolation method，and identified by morphological and molecular approaches.The host range of the strains was determined by inoculation test. Taking strain XM-6 as the test material， six common biopesticides and eight chemical pesticides were selected to screen the pesticides that could inhibit the growth of the strain by using the inhibition zone method. The pesticides with strong inhibitory effects were proportionally compounded to determine their synergistic effects. Two strains of konjac soft rot pathogens were isolated，and identified as Pectobacterium aroidearum by morphology and molecular biology. The pathogenicity test showed that the pathogenicity of strains differed from each other，and strain XM-6 was stronger than strain XM-7. The results of the host range test showed that konjac pathogens can also infect taro，sweet potato，potato，celery，carrot， white radish and cabbages. The pesticides screening test showed that the combination of 1% carvacrylol AS ： 80% Thiram WG （9：1） ）was the most effective，with a synergistic coeficient （SR）of 2.19.This experiment provides a green and scientific pesticide formulation for the control of soft-rot disease in konjac production.

ey words： Amorphophallus Blume; soft rot disease; isolation and identification； pesticide compounding; laboratory screening

魔芋（Amorphophalus Blume）是天南星科魔芋属的多年生草本植物，也是唯一可提供大量葡甘聚糖的经济作物，起源于亚洲中南半岛北部和云南南部，其始祖种是森林下层的留居者，主要有白魔芋、珠芽魔芋、花魔芋等多个种[1]。（剩余13871字）