马铃薯晚疫病数量抗性相关基因的SNP位点检测与抗病育种值分析

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Abstract：The quantitative resistance to potato late blight is controlled by multiple genes each with limited effctiveness as determined through phenotype evaluation. In order to develop novel potato germplasm with enhanced quantitative resistance，this study deployed eight parental lines to construct nine hybrid populations. Using detached leaf assays forlate blight resistance evaluation coupled with molecular marker-assisted selection， we analyzed 6O resistant and susceptible hybrid ofspring through PCR sequencing of quantitative resistance genes，identifying 19 single nucleotide polymorphism（SNP） sites across eight resistance genes.These results demonstrated that specific favorable alele （StAOS2-SNP691， StAOS2-SNP692，StGP28-SNP794，PLOX1- SNP8344） showed positive correlations with late blight resistance.The proportion of favorable homozygous allele in resistance offspring（ 9.56% ）significantly exceeded that in susceptible offspring（ 5.65% ）and parental materials （ 7.89% ）. Phylogenetic analysis based 19 SNP markers from eight genes clustered the 60 materials into three resistant and three susceptible branches.By integrating phenotypic data，breeding pedigree and resistance genesmarkers，we calculated breeding values for late blight resistance and identified superior genotypes （PC2312-3，PC2312-15，and PC2313-19） exhibiting both high breeding values and favorable SNP homozygous allele， therefore providing valuable genetic materials for improving potato resistance.

Key words： potato;late blight;quantitative resistance; SNP loci; breeding value

马铃薯（SolanumtuberosumL.）属于双子叶、茄科（Solanaceae）茄属（Solanum）一年生草本植物，起源于南美洲安第斯山脉地区[1]。（剩余15894字）