甘薯IbSAUR76基因表达特征及互作蛋白筛选

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Abstract：Sweetpotato[Ipomoea batatas （L.）Lam.] is susceptible to various biotic and abiotic stresses that adversely affect itsyield and quality.Therefore，identifying stress-responsive genesand elucidating their functional mechanisms are crucial for developing stress-resistant cultivars.As key components of auxin signaling pathways，SAUR （small auxin up RNA） genes play pivotal roles in plant growth，development，and stress adaptation.In this study，the IbSAUR76 gene was cloned and its encoded protein was demonstrated to localize to the nucleus and membrane via transient expression in tobacco.Tissue-specific expression profiling demonstrated that IbSAUR76 is ubiquitously expressed in roots，stems，leaves，petioles，and shoot tips（highest in shoot tips），as well as in storage roots at 60，75，90，and 105 days post-planting （peak at 90 days）.Analysis of the IbSAUR76 promoter sequence revealed the presence of multiple cis-acting elements associated with stress and hormone responses in the upstream region of the gene.qRT-PCR analysis demonstrated that IbSAUR76 responds toboth drought andsalt stress treatments.To elucidate the molecular mechanismsunderlying its function in sweetpotato，the IbSAUR76 bait vector was constructed for yeast two-hybrid screening against a sweetpotato cDNA library using the mating method，leading to the identification of 27 candidate interacting proteins.Two interactors IbERF2 and IbERF15，which are known to be associated with abiotic stress responses，were further validated through yeast two-hybrid reciprocal verification assays.This study enhances theunderstanding of the IbSAUR76 gene function and provides a theoretical foundation for in-depth dissection of its role and mechanism of action in sweetpotato.

Keywords：sweetpotato;IbSAUR76;auxin;interactingprotein

甘薯［Ipomoeabatatas（L.）Lam.]隶属于旋花科番薯属，是一种广泛种植的一年生蔓生草本植物[]。（剩余20952字）