甘薯IbGAPCp1在干旱和盐胁迫下的表达及上游调控因子筛选

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Abstract： Plastid glyceraldehyde-3-phosphate dehydrogenase（GAPCp）plays an important role in plant growth and development，energy metabolism，and stress response.In this study，we cloned the open reading frame（ORF） sequence of the IbGAPCpl gene from sweetpotato variety'Lizixiang'.Subcellar localization studies showed that the IbGAPCpl protein is targeted to the chloroplasts.Quantitative real-time PCR analysis showed that IbGAPCpl is ubiquitously expressed acrosssweetpotato tissues，with the highest transcripts detected in leaves.The expression of IbGAPCpl gene increased gradually with the development of sweetpotato tuberous root and reaching its peak at 105 days.In order to further explore the regulatory mechanism of IbGAPCpl gene，we cloned a 1940 bp sequence of the IbGAPCpl gene promoter，designated as Pro-IbGAPCp1.The prediction of cis-acting elements showed that Pro-IbGAPCpl contains several elements related to photoresponse，hormone response and meristem expression.Expression profiling under drought and salt stress showed that IbGAPCpl is responsive to both drought and salt stresss.Furthermore，yeast one-hybrid screening identified 35upstream transcriptional regulators interacting with the promoter region of IbGAPCp1，including ethylene-insensitive protein 2，zinc finger CCCH domain-containing protein 47 and MYB44，factors that are known to modulate plant growth and development，secondary metabolism，energy production，and stress responses.This study provides a theoretical basis for future elucidating the functional role of IbGAPCpl in sweetpotato abiotic stress tolerance.

Key words： swetpotato;plastid glyceraldehyde-3-phosphate dehydrogenase;yeast one-hybrid;abiotic stress

甘薯[Ipomoeabatatas（L.）Lam.]又名地瓜、山芋等，为旋花科双子叶块根作物，是世界上广泛种植的薯类作物之一[2]，对维护国家粮食安全具有重要作用。（剩余21234字）