甘薯不同等级脱毒苗的光合特性、产量和品质比较研究
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Abstract：The production of virus-free sweetpotato seedlings through direct field cultivation of tissuecultured sedlings or minituber-derived seedlings has become a promising propagation technology for widespread application.In order to investigate the differential effects of these two propagation methods on field performance， yield and quality across sweetpotato varieties，we conducted a comparative study using 12 sweetpotato varieties with both methodologies.The results showed that：（1） Photosynthetic characteristics -Tisse-cultured seedlings of Mingmenjinshi，Shenglibaihao，Ningzishu 4 and Pushu 32 exhibited significantly higher photosynthetic activity and pigment content compared to minituber-derived seedlings，whereas Shangshu 19 showed the opposite trend.（2）Tuber yield-Tissue-cultured seedlings of Mingmenjinshi，Shenglibaihao and Ningzishu 4 produced 30.95% ， 21.90% and 14.67% higher fresh tuber yield，respectively，but Shangshu 19 tissue-cultured seedlings yielded 10.42% less than their minituber-derived seedlings.（3） Tuber quality - Crude starch content of
Ningzishu 4 tissue-cultured seedlings，soluble sugar content of Hongyao tissue-cultured seedlings，soluble sugar content and crude protein content of Mashali tissue-cultured seedlings，crude protein content of Pushu 32 tissuecultured seedlings were all significantly lower than minituber-derived seedlings. Crude starch content of Jinhaimeixiu tisue-cultured seedlings was significantly higher than minituber-derived seedlins，but reducing sugar and soluble sugar of tisse-cultured seedlings were significantly lower than minituber-derived sedlings. Dry mattr rate of Mingmenjinshi tisue-cultured seedlings were significantly higher than minituber-derived seedlings.（4） Correlation analysis revealed distinct relationships between photosynthesis parameters and tuber quality；only water use efficiency and dry matter content showed negative correlation in tissue-cultured seedlings，whereas net photosyntheticrate，water use eficiency，and carboxylation efficiency had significant effects on the fresh potato quality of minituber-derived seedlings.This study can provide a theoretical basis for optimizing theuseoftissue-culturedand minituber-derived propagation methods fordifferent sweetpotato varieties.
Key words： sweetpotato;tissue-cultured seedling;minituber-derived seedling; photosynthesis;yield;quality
甘薯[Ipomoeabatatas（L.）Lam.]是世界第七大粮食作物[1]。（剩余13752字）
