急性氨影响副溶血弧菌的基因表达谱

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DOI：10.16424/j.cnki.cn32-1807/r.2025.04.011

[关键词]副溶血弧菌；急性氨；RNA-seq；基因表达；功能表型 [中图分类号]Q933 [文献标志码]A [文章编号] 1674-7887（2025）04-0362-08

Thegene expression profile of Vibrioparahaemolyticus exposed to acute ammonia*

WANGJian*，LUOXi*，LIXue，ZHANGYiquan，LURenfet** （Department of Clinical Laboratory，Nantong Third People's Hospital，AffiliatedNantongHospital3ofNantong University，Jiangsu 226006）

[Abstract]Objective：To investigatetheefectsofacuteammoniaonthegeneexpressonandfunctionalphenotypesof Vibrio parahaemolyticus（VP）.Methods：Usingacontrol groupwithcultureconditionsthatdidnotincludeNH4Clandanexperimental group with NH4Cl added at a final concentration of 71.68mg/L （simulating acute ammonia exposure），the RNA-seq assay was employedtoanalyzethegeneexpressionprofileofVP.Additionall，aseriesofphenotypicexperiments-includingcrystal violetstaining，motilityrelatedphenotpeassessment，andcyclicdiguanylate（c-di-GMP）contentmeasurement-werecoucted toexploretheimpactofacuteammoniaonthesefunctionalphenotypes.Results：Acuteammoniaexposureled to significant alterations inthetranscription levelsof 661 genes，including 379downregulated genesand282upregulatedgenes.These geneswereprimarilyconcentrated incellularmetabolicpathways，particularlythosecloselyrelated toaminoacidtransport andmetabolism，as wellasenergyproductionandconversion.Furthermore，acuteammoniaalsoregulatedthetranscriptionof several genes associated with biofilm formation， c -di-GMP metabolism，flagellar function，and key virulence factors.However， acuteammoniadidnotexhibitregulatoryefectsonthefunctional phenotypesthatcorrespond tothesegenes.Conclusion： Acuteammonia induced extensivetranscriptional changes inalargenumberof genes within VP，especiallthoserelated to metabolism.However，ithadnoregulatoryefectonfunctional phenotypessuchasbiofilmformation，c-di-GMPmetabolism， or motility.

[Key words] Vibrio parahaemolyticus;acute ammonia; RNA-seq; gene expression; functional phenotype

副溶血性弧菌（Vibrioparahaemolyticus，VP）广泛存在于海水和海洋生物中，是引起沿海地区细菌性食物中毒的"头号元凶"。（剩余14252字）