猪瘟病毒间接ELISA抗体检测方法的建立

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中图分类号： S852.65+9.6 文献标识码：A 文章编码：1005-8567（2025）05-0066-07

Development of Indirect ELISA Method for Antibody DetectionofClassical SwineFever Virus

YANShiqi 1，2 ，LIFukun²，QIDongmei²，LAIYuehui2*，HUANGFuqiang1*，FENGYuxin 2 ZHOU Xiaomin²，FAN Quanbao²

（1.School ofAnimal Scienceand Technology，Foshan University，Foshan Guangdong528231; 2.GuangdongWinsun Bio-Phamaceutical Co.，Ltd，Guangzhou Guangdong511356）

Abstract：Classical swine fever（CSF） caused byclassical swine fever virus （CSFV），isa highly contagious and lethal viral disease inswine，forwhich vaccinationremainsacritical preventivestrategy，antibody levelsisan important indicator for evaluating theimmuneefectof immunizedpig herds.In thisstudy，therecombinantE2Y proteinof CSFV wassuccessfully expressed using a prokaryotic expression system.The purified E2Y protein served as a coating antigen，andan indirectELISA method for detecting CSFV-specific antibodies was established throughthe systematicoptimizationof experimental conditions.Results indicated thattheoptimalcoating concentrationof the E2Y protein was 0.25μg/m L.The assay demonstrated no cross-reactivity with positive sera against common swine viruses andexhibitedhigh specificity，sensitivity，andreproducibility.Theconcordance between thismethod anda commercial ELISA kit reached 91.67% .This studysuccessfullydeveloped anindirect ELISA for CSFV antibody detection，providingatechnicalbasis forfurtheroptimizationanddevelopmentof CSFVantibodydetectionreagents.

Keywords：Classical swine fever virus；Antibodydetection;IndirectELISAmethoo

猪瘟（Classicalswinefever，CSF）是由猪瘟病毒（Classicalswinefevervirus，CSFV）引起的猪的一种高度传染性和致死性的病毒性疾病，CSFV属于黄病毒科瘟病毒属[1-2]。（剩余7099字）