高分辨质谱法筛查动物源性食品中兽药残留

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中图分类号：S859.84 文献标识码：A 文章编码：1005-8567（2025）05-0057-09

Screening of Vaterinary Drug Residues in Animal-derived Food Origin by High Resolution Mass Spectrometry

LIUYan，LILvwen，LIANGQiqi，ZHANGHuiqi，WUHongkai* （Guangdong Centre for Agricultural Products Qualityand Safety（Guangdong green food Development Center）， Guangzhou Guangdong 510523）

Abstract：This paper describes the development of mass spectrometry library and the method of screening and confirming 83 drugs and their metabolites in black-bone chicken meat，eggs，beef，and mutton using ultra-high performance liquid chromatography-quadrupole-obitrap high resolution massspectrometry（UHPLC - Q/Obitrap HRMS）. A two-step procedure was employed： homogenized samples were first extracted with 2mL aqueous solution， followed byasecond extraction using 8mLofacetonitrile.Theextractwas then passed through a purificationcolumn， reconstituted after nitrogen blow-down，and analyzed by UHPLC-Q/Obitrap.Thescreening detection limits ranged from 5.0-20μg/kg .Average recoveries across three spiked concentration levels were 60%-110% . Thismethod proved tobestraightforward，rapidandreliable，making itsuitable fortherapid screening andconfirmationanalysis of veterinary drug residues in black-bone chicken meat，eggs，beef and mutton.

Keywords：Chemical risk substances;Screening and confirming;UHPLC-Q/Obitrap HRMS

近年来，随着生活水平不断提高，人们对动物源性食品不再仅仅局限于数量，而是向着质量的需求方向转变。（剩余6172字）