干湿交替作用下水稻土DNRA菌的响应特征
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中图分类号：S511;S154.3 文献标志码：A 文章编号：1672-2043（2025）08-2169-09 doi：10.11654/jaes.2024-0745
Response characteristics of DNRA bacteria in paddy soil under wet and dry alternation
LIUJiaqi¹，ZHANGYil，SUNGeng²，NIE San'an1*
（1.College ofResources，HunanAgriculturalUniversityhangsha40128，China;2.HunanSoilandFertilizerIstute，Chagsha 410125， China）
Abstract：ThispaperaimsatstudyingtheresponseofDNRAbacteriacommunitystructuretodryandwetalternationinpaddysoils with diferentfertirsndtoalyteesposecaracteristisofsoilAbcteriatostureanagementUsingpddysoilrolg term positioning test site，three treatments of single application of chemical fertilizer（NPK）， 30% organic fertilizer + 70% chemical fertilizer（OM），andnofertlizer（CK）ascontrolwereseletedwithincubationexperimentsunderalteratingetanddry（cotuous weting，continuousfloding，andalternatingwetanddry）SoilDNAwasextractedafter23dofincubation，andhigthroughput sequencingoftheDNRAfunctionalgenenrfAwasperforedtonvestigatetheresponseofteDNRAbacterialcommunitystructuretowet anddryatemation.Theresultsshowedthat：thecompositiosandrelativeabundanceofRAbacterialcommunitiesinpaddysoilsere changedtosoeetentAttemlevel，etdryalteatiodecreasedtelatiebundaneofseudomoadotaVeucobota andChloroflexotaandincreasedtherelativeabundanceofAcidobacteriota.Continuousfloodingfavoredtherelativeabundanceof ChloroflexotabutdecreasedtherelativeabundanceofActinomycetota.TherelativeabundanceofActiomycetotawashigherunderthe continuous wetting treatment. Community α -diversity analysis showed that wet-dry alternation decreased Chaol index，Shannon index and SimpsonindexofsoilDNRAbacteria，utShaonindexandSimpsonindexwerehgherincontiuouslyfloodedsolthaninwetratmnt. Underthesamewatermanagementconditions，therewasnosignificantdiferencein ShannonindexofDNRAbacteriainpaddysoil betweendiferentfertilizationtreatments.TheresultsofprincipalcomponentanalysisshowedthatthecomunitystructureofDNRA bacteriainpaddysoil differed significantly among diffrent moisture treatments.The communitystructure and α- diversity of DNRA bacteria in paddy soil responded to the moisture changes，but the response of α- diversity to different fertilizers was not significant.We hypothesize thatthe influence of moisture managementon DNRA bacteria was greater thanthatoffertilizer management.
Keywords：paddy soil;alternatingwetand dry;DNRAbacterial ; nrfA ;community structure
硝酸盐异化还原成铵（Dissimilatorynitratereduc-tiontoammonium，DNRA）是一种厌氧呼吸过程，以NO/NO作为电子受体，将NADH还原成 NH4+ 。（剩余18139字）
