基于网络药理学和实验验证探究鲍式层孔菌多酚的抗头颈鳞癌机制

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中图分类号：R739.81 文献标志码：A DOI：10.11958/20250580

Elucidation the anti-head neck squamous cell carcinoma mechanism Phellinus baumii polyphenol based on network pharmacology experimental verification

YU Zhaoxia， MA Ben， QU Lin ， GAO Qian， N NaΔ ， ， 00， ， ; ， Stomatological ， Nankai △Corresponding Author E -mail： nina_tj@6.com

Abstract： Objective To investigate the effects Phellinus baumii polyphenol （PBP） on head neck squamous cell carcinoma （HNSCC） analyze the potential mechanism based on network pharmacology in vitro experiments. Methods Traditional Chinese Medicine Systems Pharmacology Database Analysis Platform （TCMSP）， DrugBank， GeneCards， Comparative Toxicogenomics Database （CTD） Online Mendelian nheritance in Man （OMM） database were used to screen the active components PBP potential targets PBP on HNSCC. The potential target interaction network was constructed using String database， the core targets were screened by two-step topology analysis. Enrichment analysis potential targets was performed using the DAVD database. Human HNSCC cell lines SCC-5 SCC-5 were cultured in vitro using PBP intervention 0， 5， 50mg/L ， respectively. The cell proliferation colony formation ability were detected by cell counting reagent （CCK-8） colony formation assay. Western blot assay was used to detect the expression PBP core target protein in cell lines. Results A total 80 targets were identified for 7 active components PBP， 64 which were HNSCC-related genes. Two-step topology analysis showed that hypoxia inducible factor subunit alpha （HFA）， tumor protein p5 （TP5）， AKT serine/threonine kinase （AKT）， signal transducer activator transcription （STAT）， cyclin A （CCNA） JUN proto-oncogene （JUN） were the core targets. The enrichment results suggested that PBP may play a role in HNSCC through various pathways. n vitro experiment results showed that with the increase PBP intervention concentration， the proliferation ability colony formation ability SCC-5 SCC-5 cells were significantly decreased P<0.05 ）， while the protein expression levels STAT， AKT CCNA were decreased （ P< 0.05）. Conclusion PBP can inhibit the progression HNSCC by multi-target multi-pathway.

Key words： head neck neoplasms; fungi; gene regulatory networks; cell proliferation; P. baumii polyphenol

头 颈 部 鳞 状 细 胞 癌（head neck squamouscell carcinoma，HNSCC）是世界上第 6 大常见的恶性肿瘤，因其高发病率和高死亡率对人类健康构成重大威胁［］。（剩余9248字）