骨髓间充质干细胞对脊柱损伤大鼠的影响

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关键词：骨髓间充质干细胞；脊柱损伤；运动功能；神经营养因子；PI3K/AKT/GSK-3β ⟨β -catenin通路 中图分类号：R651.2 文献标志码：A DOI：10.3969/j.issn.1007-7146.2025.06.011

Abstract：Thepurposeofthisstudywas to ivestigate the effects ofbone marrowmesenchymalstemcells（BMSCs）onmotor function recovery， neurotrophic factor expression and PI3K/AKT/GSK- 3β/β -catenin signaling pathway inrats with spinal injury. ThirtySDrats were randomlydivided into normal group，model groupandBMSCs group.Afterthe spinal injury model was established inthemodelgroupandtheBMSCs group，theBMSCs group Waslocally injected withBMSCssuspension，andthe normalgroupandthemodel group wereinjectedwiththesameamountofnormalsaline.BBBscoreandinclined plate test were used to evaluate the behavioralfunction.Hematoxylin-eosin （HE）staining Was usedtoobserve the histopathologicalchanges. Thelevels ofserum neurotrophic factor-3 （NT-3），nerve growth factor（NGF）and brain-derived neurotrophic factor（BDNF） weredetectedbyenzyme-linked immunosorbentassay.Theexpressonlevelsofpathway proteins weredetectedbyWestern blotting.Theresultsshowedthatcomparedwiththemodelgroup，MSCs treatmentsignificantlyimprovedthemotorfunction scoreandinclinedplateangleofats，educededema，erosisandiflammatoryresponseofspinalcordtissueandp-glated the level of neurotrophic factors and the expression of PI3K/AKT/GSK-3β ⟨β -catenin pathway protein.The results showed that BMSCs transplantation could promote the release of neurotrophic factors byactivating thePI3K/AKT/GSK-3β ′β -catenin pathwaytherebyimprovingtherecoveryofmotorfunctionafterspnalijuryMotordsfunctionandnsuficientererepair afterspinalinjuryareclinicalproblems.ThisstudyfocusedontherepairefectofBMSCs，exploreditseffectontherecoveryof motor function and the expresson of neurotrophic factors inrats，andthe regulatorymechanismof PI3K/AKT/GSK-3β ⟨β⟩ -catenin pathwaysoastoprovideexperimentalbasisforrevealingtheneuralrepairpotentialofMCsanddevelopingtargetedthapy strategies for spinal injury.

Key Words： bone marrow mesenchymal stemcels; spinal njury; motor function; neurotrophic factor; pI3K/AKT/GSK-3β/ β⋅ -catenin pathway

（ActaLaserBiology Sinica，2025，34（6）：569-576）

脊柱损伤是一种严重的神经系统创伤性疾病，常导致患者运动、感觉及自主神经功能的永久性障碍，给患者带来沉重的经济与心理负担[1-]。（剩余12890字）