草莓炭疽病拮抗木霉菌筛选、鉴定及功能评价

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中图分类号：S182 文献标志码：A 文章编号：1002-4026（2025）06-0060-09

开放科学（资源服务）标志码（OSID）

Screening，identification and functional evaluation of trichoderma strains

antagonistic to strawberry anthracnose

LIU Jianhua'，JIANG Yanqing'，LI Hongmei'，YANG Jianwen²，WEl Yanli1，LIU Baojun'，LI Jishun'，HU Jindong'

（1.Shandong Provincial Key Laboratoryof Applied Microbiology，Ecology Institute，Qilu Universityof Technology（Shandong AcademyofSciences），Jinan250103，China;2.ShandongTenovPesticideCo.，Ltd.，Weifang，China）

Abstract：Inresponse tothe severe incidence and management chalengesof strawberryanthracnose，this study targeted Coletotrichum siamense，thepathogenresponsiblefor thedisease.Weuseddualcultureand sealed-plateassays to screen for theefective biocontrol Trichoderma strain HB23422，whichexhibited strong antagonisticactivityagainst strawberryanthracnose，andwasidentied basedonmorphological characteristicsand molecular biologyasTrichoderma asperellum.OnPDA plates，HB23422exhibiteda strong inhibitory effect onColetotrichum siamense CM9，withan inhibition rate of more than 74.55% . Vitro leaf experiments and pot experiments showed that the spore suspension of this strain could efectively reduce the incidence of strawberry anthracnose.The strain HB 23422 grows rapidly，produces abundant spores，andhasexcelentdiseasecontroleficacy，indicatingitspotentialfordevelopmentas microbialpesticide for environment friendly control of strawberry anthracnose.

Key words：Colltotrichum siamense；Trichoderma；separation and identification；inhibition rate

草莓（Fragaria ananass Duch.）隶属于蔷薇科，是一种多年生长的草本植物。（剩余11751字）