基于非靶向代谢组学技术的忍冬不同部位差异代谢物分析

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中图分类号：R284.1 文献标志码：A 文章编号：1002-4026（2025）06-0029-13

开放科学（资源服务）标志码（OSID）

Analysis of differential metabolites in different parts of Lonicera japonica

Thunb.based on non-targeted metabolomics technology

ZHANG Xuxin1，ZHANG Luzhe'，LIN Shuqian²，ZHAO Zengcheng²，MENG Zhaoqing³， DONG Xiaodi³，CAO Guiyun³，HE Jixiang1* ，WANG Daijie4 *

（1.CollegofPharmacyhandongUniversityofTraditionalChineseMedicine，Jinan250355，China;2nstituteofPoultrySience， Shandong Academyof Agricultural Sciences，Jinan 250l0，China;3.Shandong Hongitang Pharmaceutical Group.Co，Ltd， Jinan 250102，China;4.Qilu University of Technology（Shandong Academy of Sciences），Jinan 250353，China）

Abstract：ToinvestigatethemetabolicdiferencesamongdiferentpartsofLonicerajaponicaThunb.，anontargeted metabolomics study was conducted onits stems，leaves，and flowers using ultra-high-performanceliquid chromatography tandem masspectrometry（UHPLC-MS/MS）.Intotal，73 metabolites inpositive-ion mode and 572and 572 metabolites in negative-ionmode wereidentifiedinsamples fromdiferentpartsofL.japonica Thunb.；553diferential metabolites were detected by comparing leavesandflowers；and471diferential metabolites were detected bycomparing stemsandflowers， mainly phenolicacids，aminoacidsandtheirderivatives，flavonoids，andisoflavones.Thepathwaysrelatedtodiferential metaboites mainlyincludephenylpropanoid biosynthesis，flavonoidandflavonol biosynthesis，purinemetabolism，and anthocyanin biosynthesis.This studyanalyzedthe predominant compoundsand important metabolicpathways involved in diferentpartsofL.japonicaThunb.，i.e.，thestems，leaves，andflowers，comparing themwithmedicalyusedflowers.The findingsprovides ascientific basis for the further developmentand utilization of L.japonica Thunb.resources.

Key words ∇：σ nontargeted metabolomics；Lonicera japonica Thunb.;differential metabolites；metabolic pathway

忍冬（Lonicera japonica Thunb.）是忍冬科忍冬属半常绿缠绕藤本植物，在我国的分布范围较广[1]。（剩余13930字）