脂滴特异性荧光探针的构建及细胞成像研究

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中图分类号：R378；0657.3 文献标志码：A 文章编号：1001-8751（2025）06-0395-08

Construction Lipid Droplet Specific FluorescentProbesand Studies on Cell Imaging

Liu Mei-yan， Feng Xian-hu， Chen Yong-jie， Long Chao，Su Qiang （Beijing，，， PharmacotherapySichuan 637000）

Abstract： ObjectiveTo develop a novel fluorescent probe with high selectivity for lipid droplets，excellent imaging ability，and easysynthesis.MethodDesignand synthesize afluorescent molecular probe LD-TPA with a D - π - A structure， and characterize its structure by 1H NMR， 13C NMR， and HRMS. Study the sensitivity the polarity response probe LD-TPA through spectral testing experiments.Study the stability probeLD-TPA under different pH environments.Select common inorganic salts，reactive oxygen species，amino acids，and other biomolecules as interferents to studythe anti-interference properties probe LD-TPA.Probe LD-TPA's cytotoxicity was examined using the MTTassay，and its capacity to localize to lipid droplets was examined by theuse laserconfocal microscopy for zebrafish，oleic acid stimulation，and organelle co-localization assys.ResultThe probe LD-TPA exhibits the AIE effect in a mixed solvent Dioxane /H2O and produces a regular fluorescent response to solvent polarity.The probe LD-TPA exhibits excellent characteristics such as stronganti-interferenceability，pH stability， and low cytotoxicity.Simultaneously，the probe LD-TPA has amazing dynamic imaging characteristics，including good lipid droplet localization and targeting abilities as well as thecapacityto track changes in lipid droplets cells upon stimulation byvarious external conditions.In adition，the probeLD-TPA can specifically target lipid droplets in zebrafish，therebyachievingdynamic tracking ipid droplets in living cels.ConclusionThefluorescent probe LD-TPA has potential applications in the diagnosis and treatment lipid droplet related diseases，and provides effective probe compounds for dynamic tracking lipid droplets in cells.

words： lipid droplets； fluorescent probe；AIE effect; cellimaging； pathogenic microorganisms

脂滴产生于内质网的局部脂肪，作为一种拥有脂质储存库作用的动态细胞器，对脂质运输、信号转导、膜形成和蛋白质降解等诸多生命活动具有关键影响力[1-4]。（剩余11735字）