皖南蝮蛇抑瘤组分-I通过调控RAI14抑制顺铂耐药胃癌细胞的增殖与侵袭

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Abstract：ObjectiveToevaluatetheinhibitoryefectofantitumorcomponent-IinAgkistrodonhalysvenom（AHVAC-I）on proliferationandmigrationofcisplatin-resistant gastriccancercellsandexploretheunderlyingmechanism.Methods Cisplatin-resistantMKN-28（MKN-28/DDP）cellswereobtainedbycontinuousexposureofMKN-28cellstostepwiseincreasingconcentrationsofcisplatin.MKN-28/DDPcelsweretreatedwithdiferentconcentrationsofAHVAC-Landthe changes inproliferation，migratioandvasionofthecelsereexamiedwitholonforingaCCK-8ssaoudhealing assayand Transwellassy. Westernbloting was performed toexamine theeectof AHVAC-Ionexpressionsof epithelial-mesenchymaltransition （EMT） markersof MKN-28/DDPcels;thechanges in proteinand mRNA expresionof retinoic acid induced14（RAI14）was detected with Western bloting and qRT-PCR. Results Treatment with2，4，and 8μg/mL AHVAC-Isignificantlyinibitedproliferative，migratoryandinvasionabilitiesandreducedthe expresionsofEMmarkers in MKN-28/Dcells.adih-28cells，-8/cellssodaniceasdexeionof14，ihas significantlyloweredafter treatmentwithAHVAC-1.SupplementationwithexogenousRAI4obviouslyatenuated the inhibitoryefectofAHVAC-IonproliferationandmigrationofMKN-28/DDPcels.ConclusionAHVAC-I decreases proliferation and invasion of MKN-28/DDP cells by downregulating RAI14 expression.

Keywords：Agkistrodonhalysvenom;gastriccancer;cisplatinresistance;invasion;proliferation;retinoicacidinduced14

胃癌作为高发生率和高致死率的重大疾病之一，严重威胁着人类健康1。（剩余13172字）